Placenta Regulation Genotyping QC
Generating cluster file and other resources
metadata <- read.delim("/scratch/st-dennisjk-1/wcasazza/delahaye_QC/placenta_regulatory_landscape/RootStudyConsentSet_phs001717.PlacentalRegulation.v1.p1.c1.HMB-IRB-PUB-COL-MDS/PhenotypeFiles/Placental_Regulation_Phenotypes_clean.txt",sep ="\t",skip = 1)
fam <- read.delim("/scratch/st-dennisjk-1/wcasazza/delahaye_QC/placenta_regulatory_landscape/RootStudyConsentSet_phs001717.PlacentalRegulation.v1.p1.c1.HMB-IRB-PUB-COL-MDS/genotype_qc/raw_data.fam",header=FALSE,sep="")
fam %>%
left_join(
metadata,
by = c("V2"="SUBJECT_ID")
) %>%
dplyr::select(V1,V2,Self.declared.race) %>% mutate(Self.declared.race = gsub(" |&|;","_",Self.declared.race)) %>%
write_delim(
"/scratch/st-dennisjk-1/wcasazza/delahaye_QC/placenta_regulatory_landscape/RootStudyConsentSet_phs001717.PlacentalRegulation.v1.p1.c1.HMB-IRB-PUB-COL-MDS/genotype_qc/raw_data.clst",
col_names=FALSE,
quote=FALSE
)
fam %>%
left_join(
metadata,
by = c("V2"="SUBJECT_ID")
) %>%
dplyr::select(V1,V2,Self.declared.race)metadataPre-Imputation QC RICHS
individual filtering
source /home/wcasazza/miniconda3/bin/activate
conda activate snakemake
cd /scratch/st-dennisjk-1/wcasazza/delahaye_QC/placenta_regulatory_landscape/RootStudyConsentSet_phs001717.PlacentalRegulation.v1.p1.c1.HMB-IRB-PUB-COL-MDS/
snakemake --configfile config.yaml -j4 genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.fam
cat genotype_qc/raw_data_precleaning.log
cat genotype_qc/raw_data_individual_qc.logBuilding DAG of jobs...
Nothing to be done.
Complete log: /scratch/st-dennisjk-1/wcasazza/delahaye_QC/placenta_regulatory_landscape/RootStudyConsentSet_phs001717.PlacentalRegulation.v1.p1.c1.HMB-IRB-PUB-COL-MDS/.snakemake/log/2022-04-11T161507.306554.snakemake.logPLINK v1.90b6.18 64-bit (16 Jun 2020) www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang GNU General Public License v3
Logging to genotype_qc/raw_data.geno.log.
Options in effect:
--allow-no-sex
--bfile genotype_qc/raw_data
--geno 0.05
--make-bed
--out genotype_qc/raw_data.geno
--set-hh-missing
192030 MB RAM detected; reserving 96015 MB for main workspace.
2391739 variants loaded from .bim file.
302 people (152 males, 150 females) loaded from .fam.
Using 1 thread (no multithreaded calculations invoked).
Before main variant filters, 302 founders and 0 nonfounders present.
Calculating allele frequencies... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99% done.
Total genotyping rate is 0.981226.
102585 variants removed due to missing genotype data (--geno).
2289154 variants and 302 people pass filters and QC.
Note: No phenotypes present.
--make-bed to genotype_qc/raw_data.geno.bed + genotype_qc/raw_data.geno.bim +
genotype_qc/raw_data.geno.fam ... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99%done.
MAF
462876 variants removed due to minor allele threshold(s)
missingness
1 person removed due to missing genotype data (--mind).
sex
FhetPlot Fhet within Cluster
Plotting \(F_{het}\) within each reported ethnicity:
clst <- fread(paste0(BASE_DIR,"raw_data.clst"))
fhet_data <- fread(paste0(BASE_DIR,"raw_data.geno.maf.mind.sex_check.het"))
fhet_data %>%
left_join(clst,by=c("IID"="V2")) %>%
mutate(Self.declared.race=V3) %>%
ggplot(aes(F,fill=Self.declared.race)) + geom_histogram(bins=30) + theme_minimal()
IBD Analysis
source /home/wcasazza/miniconda3/bin/activate
conda activate snakemake
cd /scratch/st-dennisjk-1/wcasazza/delahaye_QC/placenta_regulatory_landscape/RootStudyConsentSet_phs001717.PlacentalRegulation.v1.p1.c1.HMB-IRB-PUB-COL-MDS/
snakemake --configfile config.yaml -j4 genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.famBuilding DAG of jobs...
Using shell: /usr/bin/bash
Provided cores: 4
Rules claiming more threads will be scaled down.
Job counts:
count jobs
1 ibd_calculation
1
[Mon Apr 11 16:15:49 2022]
rule ibd_calculation:
input: genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.bed, genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.bim, genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.fam, genotype_qc/raw_data.clst
output: genotype_qc/raw_data.genome.gz, genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.bed, genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.bim, genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.fam
jobid: 0
wildcards: dataset=genotype_qc/raw_data
Job counts:
count jobs
1 ibd_calculation
1PLINK v1.90b6.18 64-bit (16 Jun 2020) www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang GNU General Public License v3
Logging to genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ld1.maf05.log.
Options in effect:
--bfile genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter
--indep-pairwise 200 100 0.2
--maf 0.05
--make-bed
--out genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ld1.maf05
--within genotype_qc/raw_data.clst
192030 MB RAM detected; reserving 96015 MB for main workspace.
1826278 variants loaded from .bim file.
277 people (150 males, 127 females) loaded from .fam.
--within: 4 clusters loaded, covering a total of 277 people.
Using 1 thread (no multithreaded calculations invoked).
Before main variant filters, 277 founders and 0 nonfounders present.
Calculating allele frequencies... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99% done.
Total genotyping rate is 0.997271.
461254 variants removed due to minor allele threshold(s)
(--maf/--max-maf/--mac/--max-mac).
1365024 variants and 277 people pass filters and QC.
Note: No phenotypes present.
--make-bed to
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ld1.maf05.bed +
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ld1.maf05.bim +
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ld1.maf05.fam ... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99%done.
--indep-pairwise: Ignoring 3242 chromosome 0 variants.
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Pruned 41399 variants from chromosome 13, leaving 7225.
1%
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4%
5%
6%
7%
8%
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10%
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90%
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Pruned 37275 variants from chromosome 14, leaving 6799.
1%
2%
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8%
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Pruned 35550 variants from chromosome 15, leaving 6600.
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Pruned 37826 variants from chromosome 16, leaving 7286.
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Pruned 31676 variants from chromosome 17, leaving 6807.
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Pruned 34385 variants from chromosome 18, leaving 6542.
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Pruned 22549 variants from chromosome 19, leaving 5362.
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Pruned 27907 variants from chromosome 20, leaving 5691.
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Pruned 16232 variants from chromosome 21, leaving 3192.
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Pruned 17525 variants from chromosome 22, leaving 3435.
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Pruned 30292 variants from chromosome 23, leaving 4513.
18%
37%
59%
78%
Pruned 492 variants from chromosome 24, leaving 6.
29%
58%
87%
Pruned 133 variants from chromosome 25, leaving 208.
Pruned 18 variants from chromosome 26, leaving 13.
Pruning complete. 1162074 of 1361782 variants removed.
Writing...
Marker lists written to
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ld1.maf05.prune.in and
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ld1.maf05.prune.out .
PLINK v1.90b6.18 64-bit (16 Jun 2020) www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang GNU General Public License v3
Logging to genotype_qc/raw_data_Hispanic_Non-Hispanic_White.log.
Options in effect:
--bfile genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ld1.maf05
--fst
--keep-cluster-names Hispanic Non-Hispanic_White
--out genotype_qc/raw_data_Hispanic_Non-Hispanic_White
--within genotype_qc/raw_data.clst
192030 MB RAM detected; reserving 96015 MB for main workspace.
1365024 variants loaded from .bim file.
277 people (150 males, 127 females) loaded from .fam.
--within: 2 clusters loaded, covering a total of 163 people.
114 people removed by cluster filter(s).
Using 1 thread (no multithreaded calculations invoked).
Before main variant filters, 163 founders and 0 nonfounders present.
Calculating allele frequencies... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99% done.
Total genotyping rate in remaining samples is 0.99662.
1365024 variants and 163 people pass filters and QC.
Note: No phenotypes present.
Writing --fst report (2 populations) to
genotype_qc/raw_data_Hispanic_Non-Hispanic_White.fst ... 0%0%1%1%2%2%3%3%4%4%5%5%6%6%7%7%8%8%9%10%10%11%11%12%12%13%13%14%14%15%15%16%16%17%17%18%18%19%20%20%21%21%22%22%23%23%24%24%25%25%26%26%27%27%28%28%29%30%30%31%31%32%32%33%33%34%34%35%35%36%36%37%37%38%38%39%40%40%41%41%42%42%43%43%44%44%45%45%46%46%47%47%48%48%49%50%50%51%51%52%52%53%53%54%54%55%55%56%56%57%57%58%58%59%60%60%61%61%62%62%63%63%64%64%65%65%66%66%67%67%68%68%69%70%70%71%71%72%72%73%73%74%74%75%75%76%76%77%77%78%78%79%80%80%81%81%82%82%83%83%84%84%85%85%86%86%87%87%88%88%89%90%90%91%91%92%92%93%93%94%94%95%95%96%96%97%97%98%98%99%done.
1329690 markers with valid Fst estimates.
Mean Fst estimate: 0.0136226
Weighted Fst estimate: 0.0143246
PLINK v1.90b6.18 64-bit (16 Jun 2020) www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang GNU General Public License v3
Logging to genotype_qc/raw_data_Hispanic_Asian__amp__Pacific_Islander.log.
Options in effect:
--bfile genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ld1.maf05
--fst
--keep-cluster-names Hispanic Asian__amp__Pacific_Islander
--out genotype_qc/raw_data_Hispanic_Asian__amp__Pacific_Islander
--within genotype_qc/raw_data.clst
192030 MB RAM detected; reserving 96015 MB for main workspace.
1365024 variants loaded from .bim file.
277 people (150 males, 127 females) loaded from .fam.
--within: 2 clusters loaded, covering a total of 133 people.
144 people removed by cluster filter(s).
Using 1 thread (no multithreaded calculations invoked).
Before main variant filters, 133 founders and 0 nonfounders present.
Calculating allele frequencies... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99% done.
Total genotyping rate in remaining samples is 0.99697.
1365024 variants and 133 people pass filters and QC.
Note: No phenotypes present.
Writing --fst report (2 populations) to
genotype_qc/raw_data_Hispanic_Asian__amp__Pacific_Islander.fst ... 0%0%1%1%2%2%3%3%4%4%5%5%6%6%7%7%8%8%9%10%10%11%11%12%12%13%13%14%14%15%15%16%16%17%17%18%18%19%20%20%21%21%22%22%23%23%24%24%25%25%26%26%27%27%28%28%29%30%30%31%31%32%32%33%33%34%34%35%35%36%36%37%37%38%38%39%40%40%41%41%42%42%43%43%44%44%45%45%46%46%47%47%48%48%49%50%50%51%51%52%52%53%53%54%54%55%55%56%56%57%57%58%58%59%60%60%61%61%62%62%63%63%64%64%65%65%66%66%67%67%68%68%69%70%70%71%71%72%72%73%73%74%74%75%75%76%76%77%77%78%78%79%80%80%81%81%82%82%83%83%84%84%85%85%86%86%87%87%88%88%89%90%90%91%91%92%92%93%93%94%94%95%95%96%96%97%97%98%98%99%done.
1329689 markers with valid Fst estimates (1 excluded).
Mean Fst estimate: 0.0287164
Weighted Fst estimate: 0.0325687
PLINK v1.90b6.18 64-bit (16 Jun 2020) www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang GNU General Public License v3
Logging to genotype_qc/raw_data_Hispanic_Non-Hispanic_Black.log.
Options in effect:
--bfile genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ld1.maf05
--fst
--keep-cluster-names Hispanic Non-Hispanic_Black
--out genotype_qc/raw_data_Hispanic_Non-Hispanic_Black
--within genotype_qc/raw_data.clst
192030 MB RAM detected; reserving 96015 MB for main workspace.
1365024 variants loaded from .bim file.
277 people (150 males, 127 females) loaded from .fam.
--within: 2 clusters loaded, covering a total of 165 people.
112 people removed by cluster filter(s).
Using 1 thread (no multithreaded calculations invoked).
Before main variant filters, 165 founders and 0 nonfounders present.
Calculating allele frequencies... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99% done.
Total genotyping rate in remaining samples is 0.997024.
1365024 variants and 165 people pass filters and QC.
Note: No phenotypes present.
Writing --fst report (2 populations) to
genotype_qc/raw_data_Hispanic_Non-Hispanic_Black.fst ... 0%0%1%1%2%2%3%3%4%4%5%5%6%6%7%7%8%8%9%10%10%11%11%12%12%13%13%14%14%15%15%16%16%17%17%18%18%19%20%20%21%21%22%22%23%23%24%24%25%25%26%26%27%27%28%28%29%30%30%31%31%32%32%33%33%34%34%35%35%36%36%37%37%38%38%39%40%40%41%41%42%42%43%43%44%44%45%45%46%46%47%47%48%48%49%50%50%51%51%52%52%53%53%54%54%55%55%56%56%57%57%58%58%59%60%60%61%61%62%62%63%63%64%64%65%65%66%66%67%67%68%68%69%70%70%71%71%72%72%73%73%74%74%75%75%76%76%77%77%78%78%79%80%80%81%81%82%82%83%83%84%84%85%85%86%86%87%87%88%88%89%90%90%91%91%92%92%93%93%94%94%95%95%96%96%97%97%98%98%99%done.
1329689 markers with valid Fst estimates (1 excluded).
Mean Fst estimate: 0.0416879
Weighted Fst estimate: 0.0484132
PLINK v1.90b6.18 64-bit (16 Jun 2020) www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang GNU General Public License v3
Logging to genotype_qc/raw_data_Non-Hispanic_White_Asian__amp__Pacific_Islander.log.
Options in effect:
--bfile genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ld1.maf05
--fst
--keep-cluster-names Non-Hispanic_White Asian__amp__Pacific_Islander
--out genotype_qc/raw_data_Non-Hispanic_White_Asian__amp__Pacific_Islander
--within genotype_qc/raw_data.clst
192030 MB RAM detected; reserving 96015 MB for main workspace.
1365024 variants loaded from .bim file.
277 people (150 males, 127 females) loaded from .fam.
--within: 2 clusters loaded, covering a total of 112 people.
165 people removed by cluster filter(s).
Using 1 thread (no multithreaded calculations invoked).
Before main variant filters, 112 founders and 0 nonfounders present.
Calculating allele frequencies... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99% done.
Total genotyping rate in remaining samples is 0.996625.
1365024 variants and 112 people pass filters and QC.
Note: No phenotypes present.
Writing --fst report (2 populations) to
genotype_qc/raw_data_Non-Hispanic_White_Asian__amp__Pacific_Islander.fst ...
0%0%1%1%2%2%3%3%4%4%5%5%6%6%7%7%8%8%9%10%10%11%11%12%12%13%13%14%14%15%15%16%16%17%17%18%18%19%20%20%21%21%22%22%23%23%24%24%25%25%26%26%27%27%28%28%29%30%30%31%31%32%32%33%33%34%34%35%35%36%36%37%37%38%38%39%40%40%41%41%42%42%43%43%44%44%45%45%46%46%47%47%48%48%49%50%50%51%51%52%52%53%53%54%54%55%55%56%56%57%57%58%58%59%60%60%61%61%62%62%63%63%64%64%65%65%66%66%67%67%68%68%69%70%70%71%71%72%72%73%73%74%74%75%75%76%76%77%77%78%78%79%80%80%81%81%82%82%83%83%84%84%85%85%86%86%87%87%88%88%89%90%90%91%91%92%92%93%93%94%94%95%95%96%96%97%97%98%98%99%done.
1322482 markers with valid Fst estimates (7208 excluded).
Mean Fst estimate: 0.0398269
Weighted Fst estimate: 0.0480116
PLINK v1.90b6.18 64-bit (16 Jun 2020) www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang GNU General Public License v3
Logging to genotype_qc/raw_data_Non-Hispanic_White_Non-Hispanic_Black.log.
Options in effect:
--bfile genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ld1.maf05
--fst
--keep-cluster-names Non-Hispanic_White Non-Hispanic_Black
--out genotype_qc/raw_data_Non-Hispanic_White_Non-Hispanic_Black
--within genotype_qc/raw_data.clst
192030 MB RAM detected; reserving 96015 MB for main workspace.
1365024 variants loaded from .bim file.
277 people (150 males, 127 females) loaded from .fam.
--within: 2 clusters loaded, covering a total of 144 people.
133 people removed by cluster filter(s).
Using 1 thread (no multithreaded calculations invoked).
Before main variant filters, 144 founders and 0 nonfounders present.
Calculating allele frequencies... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99% done.
Total genotyping rate in remaining samples is 0.996765.
1365024 variants and 144 people pass filters and QC.
Note: No phenotypes present.
Writing --fst report (2 populations) to
genotype_qc/raw_data_Non-Hispanic_White_Non-Hispanic_Black.fst ... 0%0%1%1%2%2%3%3%4%4%5%5%6%6%7%7%8%8%9%10%10%11%11%12%12%13%13%14%14%15%15%16%16%17%17%18%18%19%20%20%21%21%22%22%23%23%24%24%25%25%26%26%27%27%28%28%29%30%30%31%31%32%32%33%33%34%34%35%35%36%36%37%37%38%38%39%40%40%41%41%42%42%43%43%44%44%45%45%46%46%47%47%48%48%49%50%50%51%51%52%52%53%53%54%54%55%55%56%56%57%57%58%58%59%60%60%61%61%62%62%63%63%64%64%65%65%66%66%67%67%68%68%69%70%70%71%71%72%72%73%73%74%74%75%75%76%76%77%77%78%78%79%80%80%81%81%82%82%83%83%84%84%85%85%86%86%87%87%88%88%89%90%90%91%91%92%92%93%93%94%94%95%95%96%96%97%97%98%98%99%done.
1329659 markers with valid Fst estimates (31 excluded).
Mean Fst estimate: 0.0713451
Weighted Fst estimate: 0.0843464
PLINK v1.90b6.18 64-bit (16 Jun 2020) www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang GNU General Public License v3
Logging to genotype_qc/raw_data_Asian__amp__Pacific_Islander_Non-Hispanic_Black.log.
Options in effect:
--bfile genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ld1.maf05
--fst
--keep-cluster-names Asian__amp__Pacific_Islander Non-Hispanic_Black
--out genotype_qc/raw_data_Asian__amp__Pacific_Islander_Non-Hispanic_Black
--within genotype_qc/raw_data.clst
192030 MB RAM detected; reserving 96015 MB for main workspace.
1365024 variants loaded from .bim file.
277 people (150 males, 127 females) loaded from .fam.
--within: 2 clusters loaded, covering a total of 114 people.
163 people removed by cluster filter(s).
Using 1 thread (no multithreaded calculations invoked).
Before main variant filters, 114 founders and 0 nonfounders present.
Calculating allele frequencies... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99% done.
Total genotyping rate in remaining samples is 0.997211.
1365024 variants and 114 people pass filters and QC.
Note: No phenotypes present.
Writing --fst report (2 populations) to
genotype_qc/raw_data_Asian__amp__Pacific_Islander_Non-Hispanic_Black.fst ...
0%0%1%1%2%2%3%3%4%4%5%5%6%6%7%7%8%8%9%10%10%11%11%12%12%13%13%14%14%15%15%16%16%17%17%18%18%19%20%20%21%21%22%22%23%23%24%24%25%25%26%26%27%27%28%28%29%30%30%31%31%32%32%33%33%34%34%35%35%36%36%37%37%38%38%39%40%40%41%41%42%42%43%43%44%44%45%45%46%46%47%47%48%48%49%50%50%51%51%52%52%53%53%54%54%55%55%56%56%57%57%58%58%59%60%60%61%61%62%62%63%63%64%64%65%65%66%66%67%67%68%68%69%70%70%71%71%72%72%73%73%74%74%75%75%76%76%77%77%78%78%79%80%80%81%81%82%82%83%83%84%84%85%85%86%86%87%87%88%88%89%90%90%91%91%92%92%93%93%94%94%95%95%96%96%97%97%98%98%99%done.
1329634 markers with valid Fst estimates (56 excluded).
Mean Fst estimate: 0.0799649
Weighted Fst estimate: 0.0968232
PLINK v1.90b6.18 64-bit (16 Jun 2020) www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang GNU General Public License v3
Logging to genotype_qc/raw_data.log.
Options in effect:
--bfile genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ld1.maf05
--exclude genotype_qc/raw_data_fst_remove.txt
--genome gz full unbounded nudge
--out genotype_qc/raw_data
192030 MB RAM detected; reserving 96015 MB for main workspace.
1365024 variants loaded from .bim file.
277 people (150 males, 127 females) loaded from .fam.
--exclude: 1107328 variants remaining.
Using up to 31 threads (change this with --threads).
Before main variant filters, 277 founders and 0 nonfounders present.
Calculating allele frequencies... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99% done.
Total genotyping rate is 0.996781.
1107328 variants and 277 people pass filters and QC.
Note: No phenotypes present.
Excluding 35334 variants on non-autosomes from IBD calculation.
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IBD calculations complete.
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Finished writing genotype_qc/raw_data.genome.gz .
PLINK v1.90b6.18 64-bit (16 Jun 2020) www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang GNU General Public License v3
Logging to genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.log.
Options in effect:
--bfile genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter
--make-bed
--out genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter
--remove genotype_qc/raw_data_ibd_remove.txt
192030 MB RAM detected; reserving 96015 MB for main workspace.
1826278 variants loaded from .bim file.
277 people (150 males, 127 females) loaded from .fam.
--remove: 277 people remaining.
Using 1 thread (no multithreaded calculations invoked).
Before main variant filters, 277 founders and 0 nonfounders present.
Calculating allele frequencies... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99% done.
Total genotyping rate is 0.997271.
1826278 variants and 277 people pass filters and QC.
Note: No phenotypes present.
--make-bed to
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.bed +
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.bim +
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.fam ...
0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99%done.[Mon Apr 11 16:16:09 2022]
Finished job 0.
1 of 1 steps (100%) done
Complete log: /scratch/st-dennisjk-1/wcasazza/delahaye_QC/placenta_regulatory_landscape/RootStudyConsentSet_phs001717.PlacentalRegulation.v1.p1.c1.HMB-IRB-PUB-COL-MDS/.snakemake/log/2022-04-11T161547.900910.snakemake.logsystem(sprintf("gunzip %s ",paste0(BASE_DIR,"raw_data.genome.gz")))gzip: /scratch/st-dennisjk-1/wcasazza/delahaye_QC/placenta_regulatory_landscape/RootStudyConsentSet_phs001717.PlacentalRegulation.v1.p1.c1.HMB-IRB-PUB-COL-MDS/genotype_qc/raw_data.genome already exists; not overwrittenibd_report <- fread(paste0(BASE_DIR,"raw_data.genome"))
ggplot(ibd_report, aes(PI_HAT)) + geom_histogram(bins=100) + theme_minimal()
ibd_report[PI_HAT > 0.2]PCA
source /home/wcasazza/miniconda3/bin/activate
conda activate snakemake
cd /scratch/st-dennisjk-1/wcasazza/delahaye_QC/placenta_regulatory_landscape/RootStudyConsentSet_phs001717.PlacentalRegulation.v1.p1.c1.HMB-IRB-PUB-COL-MDS/
snakemake --configfile config.yaml -j4 genotype_qc/raw_data.1kg_pca.pcsBuilding DAG of jobs...
Using shell: /usr/bin/bash
Provided cores: 4
Rules claiming more threads will be scaled down.
Job counts:
count jobs
1 pc_project
1 pre_ancestry_pca
2
[Mon Apr 11 16:17:17 2022]
rule pre_ancestry_pca:
input: genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.bed, genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.bim, genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.fam
output: genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.pre_pca.bed, genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.pre_pca.bim, genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.pre_pca.fam, genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.eigenvec, genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.eigenval
jobid: 1
wildcards: dataset=genotype_qc/raw_data
Job counts:
count jobs
1 pre_ancestry_pca
1PLINK v1.90b6.18 64-bit (16 Jun 2020) www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang GNU General Public License v3
Logging to genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.autosome.log.
Options in effect:
--autosome
--bfile genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter
--maf 0.05
--make-bed
--out genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.autosome
192030 MB RAM detected; reserving 96015 MB for main workspace.
1776767 out of 1826278 variants loaded from .bim file.
277 people (150 males, 127 females) loaded from .fam.
Using 1 thread (no multithreaded calculations invoked).
Before main variant filters, 277 founders and 0 nonfounders present.
Calculating allele frequencies... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99% done.
Total genotyping rate is 0.997636.
450660 variants removed due to minor allele threshold(s)
(--maf/--max-maf/--mac/--max-mac).
1326107 variants and 277 people pass filters and QC.
Note: No phenotypes present.
--make-bed to
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.autosome.bed
+
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.autosome.bim
+
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.autosome.fam
... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99%done.
PLINK v1.90b6.18 64-bit (16 Jun 2020) www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang GNU General Public License v3
Logging to genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.autosome.exclude_regions.log.
Options in effect:
--bfile genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.autosome
--exclude range genotype_qc/raw_data.pca_to_remove.txt
--make-bed
--out genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.autosome.exclude_regions
192030 MB RAM detected; reserving 96015 MB for main workspace.
1326107 variants loaded from .bim file.
277 people (150 males, 127 females) loaded from .fam.
--exclude range: 38938 variants excluded.
--exclude range: 1287169 variants remaining.
Using 1 thread (no multithreaded calculations invoked).
Before main variant filters, 277 founders and 0 nonfounders present.
Calculating allele frequencies... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99% done.
Total genotyping rate is 0.997337.
1287169 variants and 277 people pass filters and QC.
Note: No phenotypes present.
--make-bed to
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.autosome.exclude_regions.bed
+
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.autosome.exclude_regions.bim
+
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.autosome.exclude_regions.fam
... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99%done.
PLINK v1.90b6.18 64-bit (16 Jun 2020) www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang GNU General Public License v3
Logging to genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.autosome.exclude_regions.LD_1.log.
Options in effect:
--bfile genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.autosome.exclude_regions
--indep-pairwise 200 100 0.2
--make-bed
--out genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.autosome.exclude_regions.LD_1
192030 MB RAM detected; reserving 96015 MB for main workspace.
1287169 variants loaded from .bim file.
277 people (150 males, 127 females) loaded from .fam.
Using 1 thread (no multithreaded calculations invoked).
Before main variant filters, 277 founders and 0 nonfounders present.
Calculating allele frequencies... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99% done.
Total genotyping rate is 0.997337.
1287169 variants and 277 people pass filters and QC.
Note: No phenotypes present.
--make-bed to
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.autosome.exclude_regions.LD_1.bed
+
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.autosome.exclude_regions.LD_1.bim
+
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.autosome.exclude_regions.LD_1.fam
... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99%done.
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Pruned 86457 variants from chromosome 1, leaving 15064.
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Pruned 89593 variants from chromosome 2, leaving 14119.
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Pruned 77285 variants from chromosome 3, leaving 13012.
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Pruned 74712 variants from chromosome 4, leaving 11932.
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Pruned 67454 variants from chromosome 5, leaving 11279.
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Pruned 63178 variants from chromosome 6, leaving 10777.
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Pruned 61530 variants from chromosome 7, leaving 10310.
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Pruned 57456 variants from chromosome 8, leaving 9208.
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Pruned 51272 variants from chromosome 9, leaving 9005.
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Pruned 58083 variants from chromosome 10, leaving 10026.
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Pruned 53202 variants from chromosome 11, leaving 9023.
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Pruned 53126 variants from chromosome 12, leaving 9423.
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Pruned 41399 variants from chromosome 13, leaving 7225.
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Pruned 37275 variants from chromosome 14, leaving 6799.
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Pruned 35550 variants from chromosome 15, leaving 6600.
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Pruned 37826 variants from chromosome 16, leaving 7286.
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Pruned 31676 variants from chromosome 17, leaving 6807.
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Pruned 34385 variants from chromosome 18, leaving 6542.
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Pruned 22549 variants from chromosome 19, leaving 5362.
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Pruned 27337 variants from chromosome 20, leaving 5641.
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Pruned 16232 variants from chromosome 21, leaving 3192.
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Pruned 17525 variants from chromosome 22, leaving 3435.
Pruning complete. 1095102 of 1287169 variants removed.
Writing...
Marker lists written to
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.autosome.exclude_regions.LD_1.prune.in
and
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.autosome.exclude_regions.LD_1.prune.out
.
PLINK v1.90b6.18 64-bit (16 Jun 2020) www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang GNU General Public License v3
Logging to genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.pre_pca.log.
Options in effect:
--bfile genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.autosome.exclude_regions.LD_1
--indep-pairwise 200 100 0.2
--make-bed
--out genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.pre_pca
192030 MB RAM detected; reserving 96015 MB for main workspace.
1287169 variants loaded from .bim file.
277 people (150 males, 127 females) loaded from .fam.
Using 1 thread (no multithreaded calculations invoked).
Before main variant filters, 277 founders and 0 nonfounders present.
Calculating allele frequencies... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99% done.
Total genotyping rate is 0.997337.
1287169 variants and 277 people pass filters and QC.
Note: No phenotypes present.
--make-bed to
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.pre_pca.bed
+
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.pre_pca.bim
+
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.pre_pca.fam
... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99%done.
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Pruned 77285 variants from chromosome 3, leaving 13012.
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Pruned 67454 variants from chromosome 5, leaving 11279.
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Pruned 37275 variants from chromosome 14, leaving 6799.
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Pruned 35550 variants from chromosome 15, leaving 6600.
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Pruned 37826 variants from chromosome 16, leaving 7286.
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Pruned 31676 variants from chromosome 17, leaving 6807.
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Pruned 34385 variants from chromosome 18, leaving 6542.
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Pruned 22549 variants from chromosome 19, leaving 5362.
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Pruned 16232 variants from chromosome 21, leaving 3192.
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Pruned 17525 variants from chromosome 22, leaving 3435.
Pruning complete. 1095102 of 1287169 variants removed.
Writing...
Marker lists written to
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.pre_pca.prune.in
and
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.pre_pca.prune.out
.
PLINK v1.90b6.18 64-bit (16 Jun 2020) www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang GNU General Public License v3
Logging to genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.log.
Options in effect:
--bfile genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.pre_pca
--out genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter
--pca
192030 MB RAM detected; reserving 96015 MB for main workspace.
1287169 variants loaded from .bim file.
277 people (150 males, 127 females) loaded from .fam.
Using up to 31 threads (change this with --threads).
Before main variant filters, 277 founders and 0 nonfounders present.
Calculating allele frequencies... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99% done.
Total genotyping rate is 0.997337.
1287169 variants and 277 people pass filters and QC.
Note: No phenotypes present.
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Relationship matrix calculation complete.
[extracting eigenvalues and eigenvectors]
--pca: Results saved to
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.eigenval and
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.eigenvec .[Mon Apr 11 16:17:36 2022]
Finished job 1.
1 of 2 steps (50%) done
[Mon Apr 11 16:17:36 2022]
rule pc_project:
input: genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.pre_pca.bed, genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.pre_pca.bim, genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.pre_pca.fam
output: genotype_qc/raw_data.1kg_pca.pcs, genotype_qc/raw_data.kg_projection.txt
jobid: 0
wildcards: dataset=genotype_qc/raw_data
Job counts:
count jobs
1 pc_project
1
Loading required package: data.tablePLINK v1.90b6.18 64-bit (16 Jun 2020) www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang GNU General Public License v3
Logging to genotype_qc/raw_data.kg_matched.log.
Options in effect:
--bfile genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.pre_pca
--extract genotype_qc/raw_data_filter.txt
--make-bed
--out genotype_qc/raw_data.kg_matched
192030 MB RAM detected; reserving 96015 MB for main workspace.
1287169 variants loaded from .bim file.
277 people (150 males, 127 females) loaded from .fam.
--extract: 1208095 variants remaining.
Using 1 thread (no multithreaded calculations invoked).
Before main variant filters, 277 founders and 0 nonfounders present.
Calculating allele frequencies... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99% done.
Total genotyping rate is 0.997343.
1208095 variants and 277 people pass filters and QC.
Note: No phenotypes present.
--make-bed to genotype_qc/raw_data.kg_matched.bed +
genotype_qc/raw_data.kg_matched.bim + genotype_qc/raw_data.kg_matched.fam ...
0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99%done.
PLINK v1.90b6.18 64-bit (16 Jun 2020) www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang GNU General Public License v3
Logging to genotype_qc/raw_data.kg_matched.unified.log.
Options in effect:
--bed genotype_qc/raw_data.kg_matched.bed
--bim genotype_qc/raw_data_matched.bim
--fam genotype_qc/raw_data.kg_matched.fam
--make-bed
--out genotype_qc/raw_data.kg_matched.unified
192030 MB RAM detected; reserving 96015 MB for main workspace.
1208095 variants loaded from .bim file.
277 people (150 males, 127 females) loaded from .fam.
Using 1 thread (no multithreaded calculations invoked).
Before main variant filters, 277 founders and 0 nonfounders present.
Calculating allele frequencies... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99% done.
Total genotyping rate is 0.997343.
1208095 variants and 277 people pass filters and QC.
Note: No phenotypes present.
--make-bed to genotype_qc/raw_data.kg_matched.unified.bed +
genotype_qc/raw_data.kg_matched.unified.bim +
genotype_qc/raw_data.kg_matched.unified.fam ... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99%done.
PLINK v1.90b6.18 64-bit (16 Jun 2020) www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang GNU General Public License v3
Logging to genotype_qc/raw_data.1kg_pca.log.
Options in effect:
--bfile /arc/project/st-dennisjk-1/shared/data/1000G_phase3_plink/all_phase3_biallelic_autosome_pre_pca
--extract genotype_qc/raw_data.kg_matched.unified.bim
--make-bed
--out genotype_qc/raw_data.1kg_pca
192030 MB RAM detected; reserving 96015 MB for main workspace.
6602014 variants loaded from .bim file.
2504 people (1233 males, 1271 females) loaded from .fam.
--extract: 1204954 variants remaining.Warning: At least 3141 duplicate IDs in --extract file.Using 1 thread (no multithreaded calculations invoked).
Before main variant filters, 2497 founders and 7 nonfounders present.
Calculating allele frequencies... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99% done.
1204954 variants and 2504 people pass filters and QC.
Note: No phenotypes present.
--make-bed to genotype_qc/raw_data.1kg_pca.bed +
genotype_qc/raw_data.1kg_pca.bim + genotype_qc/raw_data.1kg_pca.fam ... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99%done.
[Mon Apr 11 16:18:02 2022] arguments: flashpca /arc/project/st-dennisjk-1/software/flashpca/flashpca_x86-64 --bfile genotype_qc/raw_data.1kg_pca --outmeansd genotype_qc/raw_data.1kg_pca.meansd --outload genotype_qc/raw_data.1kg_pca.loadings --outpc genotype_qc/raw_data.1kg_pca.pcs
[Mon Apr 11 16:18:02 2022] Start flashpca (version 2.0)
[Mon Apr 11 16:18:02 2022] seed: 1
[Mon Apr 11 16:18:04 2022] blocksize: 89261 (1788076352 bytes per block)
[Mon Apr 11 16:18:04 2022] PCA begin
[Mon Apr 11 16:24:54 2022] PCA done
[Mon Apr 11 16:24:54 2022] Writing 10 eigenvalues to file eigenvalues.txt
[Mon Apr 11 16:24:54 2022] Writing 10 eigenvectors to file eigenvectors.txt
[Mon Apr 11 16:24:54 2022] Writing 10 PCs to file genotype_qc/raw_data.1kg_pca.pcs
[Mon Apr 11 16:24:54 2022] Writing 10 proportion variance explained to file pve.txt
[Mon Apr 11 16:24:55 2022] Writing SNP loadings to file genotype_qc/raw_data.1kg_pca.loadings
[Mon Apr 11 16:25:01 2022] Writing mean + sd file genotype_qc/raw_data.1kg_pca.meansd
[Mon Apr 11 16:25:04 2022] Goodbye!
[Mon Apr 11 16:25:04 2022] arguments: flashpca /arc/project/st-dennisjk-1/software/flashpca/flashpca_x86-64 --project --bfile genotype_qc/raw_data.kg_matched.unified --inmeansd genotype_qc/raw_data.1kg_pca.meansd --inload genotype_qc/raw_data.1kg_pca.loadings --outproj genotype_qc/raw_data.kg_projection.txt
[Mon Apr 11 16:25:04 2022] Start flashpca (version 2.0)
[Mon Apr 11 16:25:04 2022] seed: 1
[Mon Apr 11 16:25:10 2022] blocksize: 850346 (1884366736 bytes per block)
[Mon Apr 11 16:25:25 2022] Goodbye![Mon Apr 11 16:25:25 2022]
Finished job 0.
2 of 2 steps (100%) done
Complete log: /scratch/st-dennisjk-1/wcasazza/delahaye_QC/placenta_regulatory_landscape/RootStudyConsentSet_phs001717.PlacentalRegulation.v1.p1.c1.HMB-IRB-PUB-COL-MDS/.snakemake/log/2022-04-11T161716.708545.snakemake.logpcs <- fread(paste0(BASE_DIR,"raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.eigenvec"))
plot_pcs <- function(dat,pc_col1,pc_col2,pc,what,color="Self.declared.race"){
p1 <- dat %>%
inner_join(metadata,by = c("V2"="SUBJECT_ID")) %>%
mutate(Self.declared.race= as.factor(Self.declared.race)) %>%
ggplot(aes_string(pc_col1,pc_col2,color=color)) +
geom_point() +
labs(x=sprintf("PC%d",pc),y=sprintf("PC%d",pc+1)) +
theme_minimal()
p2 <- dat %>%
inner_join(metadata,by = c("V2"="SUBJECT_ID")) %>%
mutate(Self.declared.race= as.factor(Self.declared.race)) %>%
ggplot(aes_string(pc_col1,"Gestational.Age.at.Delivery",color=color)) +
geom_point() +
labs(x=sprintf("PC%d",pc)) +
ggtitle("PCA Placental Regulation vs. Age") +
theme_minimal()
if(what != ""){
print(p2)
}else{
print(p1)
}
}
plot_pcs(pcs,"V3","V4",1,"")
plot_pcs(pcs,"V4","V5",2,"")
plot_pcs(pcs,"V5","V6",3,"")
plot_pcs(pcs,"V6","V7",4,"")
plot_pcs(pcs,"V7","V8",5,"")
plot_pcs(pcs,"V3","V4",1,"age")
plot_pcs(pcs,"V4","V5",2,"age")
plot_pcs(pcs,"V5","V6",3,"age")
plot_pcs(pcs,"V6","V7",4,"age")
plot_pcs(pcs,"V7","V8",5,"age")
panel_file <- fread("/arc/project/st-dennisjk-1/shared/data/1000G_phase3_info/integrated_call_samples_v3.20130502.ALL.panel")Detected 6 column names but the data has 4 columns. Filling rows automatically. Set fill=TRUE explicitly to avoid this warning.kg_case_matched_pcs <- fread(paste0(BASE_DIR,"raw_data.1kg_pca.pcs")) %>% inner_join(panel_file, by=c("IID"="sample")) %>% select(FID,IID,contains("PC"),pop,super_pop)
cases_projected <- fread(paste0(BASE_DIR,"raw_data.kg_projection.txt"))%>% inner_join(metadata,by = c("IID"="SUBJECT_ID")) %>% select(FID,IID,contains("PC"),Self.declared.race)%>% rename(pop=Self.declared.race) %>% mutate(super_pop="Placenta_DATA")
kg_case_pcs <- rbind(kg_case_matched_pcs,cases_projected)
ggplot(kg_case_pcs,aes(PC1,PC2,color=super_pop)) + geom_point()
ggplot(kg_case_pcs,aes(PC2,PC3,color=super_pop)) + geom_point()
ggplot(kg_case_pcs,aes(PC3,PC4,color=super_pop)) + geom_point()
ggplot(kg_case_pcs,aes(PC4,PC5,color=super_pop)) + geom_point()
subset_kg_case_pcs <- kg_case_pcs%>% filter(super_pop %in% c("EUR","Placenta_DATA"))
ggplot(subset_kg_case_pcs,aes(PC1,PC2,color=pop,shape=super_pop)) + geom_point()
ggplot(subset_kg_case_pcs,aes(PC2,PC3,color=pop,shape=super_pop)) + geom_point()
ggplot(subset_kg_case_pcs,aes(PC3,PC4,color=pop,shape=super_pop)) + geom_point()
ggplot(subset_kg_case_pcs,aes(PC4,PC5,color=pop,shape=super_pop)) + geom_point()
ggplot(subset_kg_case_pcs,aes(PC5,PC6,color=pop,shape=super_pop)) + geom_point()
subset_kg_case_pcs <- kg_case_pcs%>% filter(super_pop %in% c("EUR","AFR","RICHS_DATA"))
ggplot(subset_kg_case_pcs,aes(PC1,PC2,color=pop,shape=super_pop)) + geom_point()
ggplot(subset_kg_case_pcs,aes(PC2,PC3,color=pop,shape=super_pop)) + geom_point()
ggplot(subset_kg_case_pcs,aes(PC3,PC4,color=pop,shape=super_pop)) + geom_point()
ggplot(subset_kg_case_pcs,aes(PC4,PC5,color=pop,shape=super_pop)) + geom_point()
ggplot(subset_kg_case_pcs,aes(PC5,PC6,color=pop,shape=super_pop)) + geom_point()
subset_kg_case_pcs <- kg_case_pcs%>% filter(super_pop %in% c("EUR","EAS","RICHS_DATA"))
ggplot(subset_kg_case_pcs,aes(PC1,PC2,color=pop,shape=super_pop)) + geom_point()
ggplot(subset_kg_case_pcs,aes(PC2,PC3,color=pop,shape=super_pop)) + geom_point()
ggplot(subset_kg_case_pcs,aes(PC3,PC4,color=pop,shape=super_pop)) + geom_point()
ggplot(subset_kg_case_pcs,aes(PC4,PC5,color=pop,shape=super_pop)) + geom_point()
ggplot(subset_kg_case_pcs,aes(PC5,PC6,color=pop,shape=super_pop)) + geom_point()
subset_kg_case_pcs <- kg_case_pcs%>% filter(super_pop %in% c("EAS","AFR","RICHS_DATA"))
ggplot(subset_kg_case_pcs,aes(PC1,PC2,color=pop,shape=super_pop)) + geom_point()
ggplot(subset_kg_case_pcs,aes(PC2,PC3,color=pop,shape=super_pop)) + geom_point()
ggplot(subset_kg_case_pcs,aes(PC3,PC4,color=pop,shape=super_pop)) + geom_point()
ggplot(subset_kg_case_pcs,aes(PC4,PC5,color=pop,shape=super_pop)) + geom_point()
ggplot(subset_kg_case_pcs,aes(PC5,PC6,color=pop,shape=super_pop)) + geom_point()
write_delim(data.frame(population=unique(panel_file$pop[panel_file$super_pop %in% c("EUR","EAS","AFR", "AMR")]),is_super_pop=FALSE),paste0(BASE_DIR,"raw_data.ancestry_clusters.txt"))source /home/wcasazza/miniconda3/bin/activate
conda activate snakemake
cd /scratch/st-dennisjk-1/wcasazza/delahaye_QC/placenta_regulatory_landscape/RootStudyConsentSet_phs001717.PlacentalRegulation.v1.p1.c1.HMB-IRB-PUB-COL-MDS/
snakemake --configfile config.yaml -j4 genotype_qc/raw_data_assigned_ancestry.clstBuilding DAG of jobs...
Using shell: /usr/bin/bash
Provided cores: 4
Rules claiming more threads will be scaled down.
Job counts:
count jobs
1 ancestry_assignment
1
[Mon Apr 11 16:28:22 2022]
rule ancestry_assignment:
input: genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.bed, genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.bim, genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.fam, genotype_qc/raw_data.clst, genotype_qc/raw_data.1kg_pca.pcs, genotype_qc/raw_data.kg_projection.txt, genotype_qc/raw_data.ancestry_clusters.txt
output: genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.ancestry.bed, genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.ancestry.bim, genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.ancestry.fam, genotype_qc/raw_data_assigned_ancestry.clst
jobid: 0
wildcards: dataset=genotype_qc/raw_data
Job counts:
count jobs
1 ancestry_assignment
1
── Attaching packages ────────────────────────────────── tidyverse 1.3.0 ──
✓ ggplot2 3.3.2 ✓ purrr 0.3.4
✓ tibble 3.0.3 ✓ dplyr 1.0.2
✓ tidyr 1.1.2 ✓ stringr 1.4.0
✓ readr 1.3.1 ✓ forcats 0.5.0
── Conflicts ───────────────────────────────────── tidyverse_conflicts() ──
x dplyr::filter() masks stats::filter()
x dplyr::lag() masks stats::lag() FID IID GBR_dist FIN_dist CHS_dist PUR_dist CDX_dist CLM_dist IBS_dist
1 0 8366 159.3719 121.7290 184.2651 23.68296 180.6331 27.88008 64.69827
2 0 8367 157.6871 120.2052 182.2080 24.04751 178.9303 28.08355 64.68208
3 0 8368 160.3108 122.5061 185.0398 23.46059 181.2923 27.54917 65.14541
4 0 8369 158.5116 120.9385 182.4608 24.64856 179.0270 29.06885 64.48347
5 0 8370 159.3806 121.7005 183.6830 23.73572 179.9411 27.86449 64.91959
6 0 8372 159.0562 121.5668 183.8708 23.79599 180.1285 27.98557 64.62724
7 0 8374 158.3814 120.8746 182.9505 23.97148 179.3827 28.21509 64.80498
8 0 8394 163.4300 125.2139 188.6483 22.51215 185.3728 26.42749 65.74639
9 0 8396 158.7764 121.3504 183.5576 23.96949 179.9618 28.18443 64.65434
10 0 8399 159.0722 121.2500 182.9574 24.12122 179.6719 28.34267 65.16880
11 0 8400 159.3021 121.5423 184.0548 23.51748 180.4907 27.78090 64.99217
12 0 8401 159.8204 122.0827 184.1834 23.77010 180.6446 27.88069 65.21092
13 0 8403 158.9193 121.3013 183.1677 23.92042 179.7542 28.25984 64.85312
14 0 8405 159.0931 121.6287 184.1748 23.66777 180.8990 27.72745 64.67379
15 0 8407 158.9568 120.9958 183.0535 24.19617 179.1825 28.56392 64.96039
16 0 8410 164.2841 125.6548 188.4278 22.92350 184.7567 26.84593 66.20378
17 0 8418 159.3366 121.8231 184.6063 23.69497 180.8214 27.94875 64.62930
18 0 8419 161.4727 123.4708 186.6606 23.02801 183.1052 26.92372 65.06790
19 0 8420 161.5344 123.6016 186.5672 23.06024 183.2981 27.09546 65.21035
20 0 8421 163.1341 124.7669 187.6092 22.79765 184.7147 26.48397 65.70221
21 0 8422 161.0116 123.0763 185.7109 23.21357 182.0996 27.26978 65.27060
22 0 8423 158.8230 121.2785 183.5305 23.61089 179.8729 27.82312 64.75865
23 0 8424 159.1904 121.4639 183.6182 23.70405 180.1180 27.85105 65.02937
24 0 8425 160.2629 122.3358 184.2446 24.08554 180.7553 28.25972 65.13824
25 0 8430 162.6383 124.5643 187.6444 22.78079 184.4774 26.66248 65.39078
26 0 8431 159.4680 121.9078 184.3625 23.61000 181.0212 27.80812 64.69984
27 0 8432 159.0995 121.4183 182.7643 24.41432 179.0318 28.91268 65.08417
28 0 8433 159.9268 121.9900 183.6665 23.97580 179.9094 28.28262 65.45394
29 0 8434 158.3781 120.9636 183.3162 24.19373 179.4205 28.44299 64.25972
30 0 8435 159.0377 121.4349 184.0427 23.77211 180.2522 28.12544 64.59416
31 0 8436 158.4438 120.8194 182.8600 24.14522 178.7968 28.56994 64.54365
32 0 8437 158.6717 121.1323 182.3239 24.36500 178.7267 28.64562 64.99774
33 0 8438 158.8369 121.3512 184.0210 23.62734 179.9147 27.96659 64.55847
34 0 8439 158.2261 120.5714 183.0804 24.07006 179.3477 28.27216 64.87828
35 0 8440 158.7716 121.1699 183.1166 24.09669 179.4301 28.38419 64.88525
36 0 8441 160.4810 122.6217 185.1397 23.16811 181.4693 27.24840 65.43087
37 0 8442 158.2057 120.8571 183.6374 23.68704 179.7544 27.98536 64.30636
38 0 8444 164.6446 126.1138 189.4480 22.50351 185.7794 26.31527 66.18758
39 0 8445 160.4905 122.7132 185.4784 23.40961 182.0834 27.51008 64.86891
40 0 8447 158.8378 121.3484 183.1383 24.01585 179.7897 28.25365 64.92614
41 0 8448 161.7322 123.4716 186.3182 23.48830 182.4844 27.53237 65.31132
42 0 8449 158.7736 121.2815 183.6974 23.87608 179.8954 27.91816 64.58598
43 0 8450 158.6386 121.0242 182.7468 24.17454 178.9709 28.34670 64.94458
44 0 8451 162.6117 124.3702 187.5983 22.72391 184.3214 26.48593 65.68947
45 0 8452 160.4366 122.5565 185.5911 23.18512 181.9007 27.12184 65.37558
46 0 8453 159.2332 121.5507 184.0036 23.86932 180.2723 28.19620 64.66171
47 0 8454 159.3933 121.8537 184.8202 23.07939 181.2661 27.27802 64.91661
48 0 8455 159.6563 121.8535 184.3392 23.52363 180.9711 27.49945 65.24681
49 0 8456 164.0391 125.6137 188.3622 22.76943 185.1695 26.74081 65.80675
50 0 8457 160.4299 122.5565 185.4472 23.74490 181.7796 27.91717 64.76589
51 0 8458 163.4032 125.2089 187.8634 23.29992 184.2443 27.14971 65.84133
52 0 8459 158.6170 120.7336 182.5980 24.27345 178.8247 28.60023 65.06256
53 0 8460 158.7426 121.0660 182.8531 24.22592 179.3190 28.52547 64.97369
54 0 8461 158.4474 121.0762 183.1942 23.71351 179.7300 27.90048 65.00642
55 0 8462 158.8896 121.2457 182.8567 24.51783 179.0976 28.87225 64.56873
56 0 8463 159.1225 121.4101 183.3116 24.21214 179.8046 28.39211 64.84398
57 0 8464 157.7895 120.4207 182.5296 24.08166 178.6928 28.46563 64.34896
58 0 8465 158.2143 120.8688 183.5796 23.70458 180.0384 27.90112 64.47134
59 0 8466 162.7325 124.4860 188.1793 22.84548 184.3429 26.65578 65.35075
60 0 8467 163.8832 125.2944 188.3516 22.79282 184.8576 26.66415 65.94260
61 0 8468 164.6124 126.0167 189.2328 22.92311 185.8743 26.79065 65.86911
62 0 8469 160.0796 122.3807 185.0667 23.37485 181.2571 27.60046 64.78910
63 0 8470 160.8688 122.9468 186.1215 23.13323 182.7500 26.99975 64.98057
64 0 8471 158.2634 120.6740 183.1357 24.02616 179.1512 28.47374 64.41678
65 0 8472 158.4814 120.9419 183.4862 23.79471 179.7922 28.03990 64.47211
66 0 8492 164.5582 126.0737 189.4903 22.29727 186.2087 26.04699 65.83037
67 0 8493 164.2932 125.7237 189.2587 22.57958 186.0115 26.16228 66.07294
68 0 8494 162.2229 123.9957 187.4345 23.07572 183.7108 26.97002 65.18275
69 0 8495 159.3284 121.6013 183.9164 23.52678 180.5307 27.47817 65.24326
70 0 8496 162.0987 124.0736 187.1940 22.92235 183.4906 27.06898 65.18188
71 0 8497 160.5879 122.5345 184.7460 23.79155 181.0848 28.00871 65.27266
72 0 8498 160.3293 122.6068 184.8781 23.59163 181.4417 27.73670 65.05122
73 0 8499 161.5784 123.5566 186.4674 23.49868 182.9010 27.36401 65.24788
74 0 8500 160.3413 122.7368 185.4138 23.68524 181.7105 27.86023 64.70655
75 0 8501 163.2384 124.9966 188.1875 22.80924 184.7282 26.75520 65.26035
76 0 8504 160.1363 122.4879 184.8902 23.58551 181.2381 27.85361 64.95883
77 0 8505 160.6844 122.7971 185.3999 23.22280 181.5818 27.38377 65.05882
78 0 8506 158.4355 120.6219 182.6805 24.39890 178.8708 28.69039 64.84453
79 0 8507 163.5403 125.1296 188.1075 22.78700 184.7187 26.72247 65.75816
80 0 8508 161.4435 123.3047 186.0012 23.49786 182.5354 27.47197 65.37549
81 0 8509 163.3040 124.8730 187.6853 22.96365 184.1506 26.94841 65.56873
82 0 8510 162.6600 124.1852 186.8635 23.02821 183.2391 27.07332 66.14142
83 0 8511 160.0607 122.5136 184.8470 23.81458 181.3075 28.06809 64.56416
84 0 8512 158.9657 121.4852 183.4407 24.33825 179.8391 28.61242 64.89666
85 0 8515 164.6520 126.1013 189.3938 22.87807 185.8759 26.80861 65.76498
86 0 8516 164.5149 126.1168 189.2857 22.59266 186.1080 26.22809 65.98151
87 0 8517 159.9836 122.1926 183.9936 24.12868 180.2498 28.40838 65.00130
88 0 8519 160.2195 122.3380 184.6648 23.86411 181.4560 27.90728 65.04007
89 0 8520 160.7801 122.7188 185.3054 23.27204 182.1022 27.12076 65.66219
90 0 8521 160.4224 122.7236 185.3598 23.30530 181.9138 27.23924 65.07702
91 0 8522 163.5467 125.2009 188.1791 22.87633 184.8983 26.70034 65.74332
92 0 8524 159.6791 122.3364 185.5948 23.12060 182.1305 27.36847 64.31756
93 0 8527 163.4762 125.2533 188.3182 22.81481 184.7403 26.75226 65.48199
94 0 8529 163.8842 125.5090 188.6794 22.51569 185.3377 26.27709 65.76841
95 0 8530 161.1998 123.2725 186.0929 23.30787 182.6371 27.25755 65.18135
96 0 8531 161.0235 123.0582 185.9389 23.21468 182.3004 27.21503 65.29327
97 0 8532 165.3644 126.4021 189.3276 22.76446 185.9507 26.56843 66.48294
98 0 8533 158.7645 121.0242 183.1259 24.04436 179.3490 28.25598 64.85238
99 0 8534 163.8647 125.2897 188.5698 22.88491 185.0242 26.75668 65.81350
100 0 8535 163.8632 125.4502 188.5319 22.64183 185.1455 26.55594 66.05531
101 0 8536 163.8050 125.4009 188.6129 22.69859 185.3419 26.41580 65.87786
102 0 8542 165.1246 126.2644 189.5319 22.61203 186.2068 26.48222 66.14778
103 0 8543 162.6315 124.5074 187.6037 23.08194 183.8974 27.09826 65.32460
104 0 8544 159.1969 121.7345 184.1097 23.85483 180.4851 28.31926 64.75860
105 0 8546 160.2662 122.3124 184.4991 23.74241 180.9351 27.91410 65.40980
106 0 8547 161.3999 123.4888 186.9753 22.92018 183.3481 26.88836 65.21578
107 0 8548 164.3608 125.8935 189.6198 22.50139 186.2755 26.23107 65.88816
108 0 8549 162.5444 124.2740 187.5592 22.84093 184.2607 26.62398 65.77677
109 0 8550 163.1661 124.7911 187.6741 22.76640 184.1677 26.79799 65.94431
110 0 8551 158.8920 121.1788 183.8961 23.56558 180.4329 27.51519 64.81001
111 0 8552 159.7870 121.9018 184.2797 24.02367 180.4530 28.35504 64.85986
112 0 8554 161.3306 123.4563 186.3361 23.18430 182.7099 27.22948 65.43560
113 0 8555 161.0426 123.1411 185.9154 23.36377 182.0004 27.60627 64.97436
114 0 8556 160.2839 122.6467 185.5585 23.42316 181.7438 27.60021 64.58790
115 0 8558 160.7386 122.8647 185.8117 23.40834 181.7466 27.61817 64.93789
116 0 8559 159.9070 122.0079 184.0867 24.01734 180.4994 28.29632 65.09421
117 0 8560 160.5265 122.6821 185.2288 23.43637 181.5811 27.69156 65.09067
118 0 8561 159.8545 122.2539 184.5947 23.53043 180.8256 27.79256 64.72239
119 0 8562 160.5135 122.3547 185.1656 23.69407 181.4850 27.90628 65.02909
120 0 8563 163.9495 125.5902 188.4707 22.72423 185.3464 26.66306 65.65510
121 0 8564 160.0778 122.2380 185.0945 23.45607 181.6890 27.54282 65.13222
122 0 8565 158.8618 121.3033 184.3004 23.57054 180.1575 27.89330 64.64602
123 0 8568 159.3779 121.7354 184.6376 23.69458 180.9219 27.75537 64.70356
124 0 8570 161.0132 123.2590 186.1726 23.46959 182.4222 27.70382 64.78749
125 0 8572 159.7190 122.0680 184.4671 23.66488 180.9184 27.82065 65.03746
126 0 8573 164.2754 125.7440 189.3770 22.32861 186.1054 25.94734 66.10786
127 0 8574 159.9357 121.9869 183.7687 24.08481 180.2837 28.21828 65.36005
128 0 8576 163.3723 125.2346 188.6407 22.85923 185.3106 26.69866 65.59740
129 0 8577 161.1648 123.2534 186.4103 23.15903 182.6872 27.19667 65.11971
130 0 8579 159.5005 121.6980 184.0767 23.83629 180.1013 28.23140 65.09754
131 0 8581 161.6999 123.7702 186.8659 23.07738 183.3816 26.89174 65.36503
132 0 8582 159.7473 122.0238 184.4084 23.85635 180.7406 28.04304 64.91219
133 0 8583 163.2410 124.9561 187.6957 23.00460 184.4965 26.96990 65.74432
134 0 8584 160.6021 122.8106 185.7585 23.15373 182.2886 27.12457 64.98067
135 0 8585 163.8493 125.4288 189.2480 22.33533 186.2144 26.00884 65.82695
136 0 8587 159.8576 122.0267 184.1703 24.03388 180.5675 28.25273 64.98682
137 0 8588 163.1459 124.9650 188.7128 22.19015 185.5405 25.90935 65.80496
138 0 8589 158.6475 120.9237 182.7944 24.00423 179.2373 28.15616 65.18613
139 0 8590 161.2616 123.4426 186.1270 23.31517 182.4109 27.56074 65.26552
140 0 8591 160.3296 122.5289 184.9086 23.63205 181.3967 27.76502 65.21325
141 0 8592 161.0687 123.2270 186.6458 22.87827 183.0850 26.93925 65.03758
142 0 8593 164.6106 126.0521 189.2417 22.40824 185.9718 26.28672 66.24502
143 0 8595 163.6734 125.1719 187.9137 22.70508 184.8560 26.49506 66.15531
144 0 8604 158.0752 120.4428 182.6435 23.92011 179.1162 28.22044 64.70683
145 0 8605 159.4090 121.8508 184.1772 23.52434 180.4659 27.66881 64.98442
146 0 8606 162.4368 124.7483 188.4070 22.13805 185.2721 26.03090 65.42707
147 0 8607 164.0331 125.7247 188.7069 22.86342 185.1556 26.79915 65.98743
148 0 8608 164.1721 125.6394 188.9425 22.92829 185.5572 26.39239 65.85139
149 0 8609 162.7723 124.9165 188.4251 22.30069 185.4971 25.84951 65.70822
150 0 8610 162.1678 124.2875 187.4658 22.47003 184.1051 26.37653 65.35499
151 0 8611 160.2721 122.4095 185.3801 23.14594 181.6333 27.32116 65.30476
152 0 8612 160.2778 122.5541 185.6487 23.09914 182.0620 27.03301 65.08185
153 0 8613 159.7025 122.0620 184.1883 24.25937 180.6191 28.47702 64.60565
154 0 8614 163.6541 125.2990 188.8420 22.45375 185.3969 26.35312 65.80652
155 0 8616 162.3498 124.2316 186.6272 23.19420 182.9729 27.21873 65.56850
156 0 8617 163.1069 124.8358 188.1352 22.90367 184.3301 26.92589 65.56188
157 0 8618 163.8576 125.4918 189.0765 22.26087 186.1238 26.07976 65.87271
158 0 TB8683 161.0244 122.9127 185.1226 23.90730 181.5762 28.21943 65.27922
159 0 TB8684 160.2739 122.2426 184.3415 23.66887 180.6296 27.86031 65.45975
160 0 TB8685 160.7314 122.6876 185.4174 23.42627 181.5751 27.45301 65.08126
161 0 TB8734 160.1303 122.4600 185.3536 23.30258 181.7387 27.38899 64.85990
162 0 TB8735 160.2798 122.4553 184.7950 23.61880 181.4495 27.74439 65.21537
163 0 TB8736 159.1624 121.3863 183.1320 24.07868 179.4605 28.34394 64.99501
164 0 TB8737 159.6879 121.7773 184.6745 23.60774 180.5842 27.88624 64.91380
165 0 TB8738 159.2108 121.4903 183.9613 23.78475 180.1202 28.04484 64.94256
166 0 TB8739 161.0305 123.1453 185.9480 23.71553 182.2000 27.81439 64.79849
167 0 TB8740 160.0754 122.3293 184.9339 23.46190 181.2624 27.62801 64.94094
168 0 TB8741 158.5611 121.0719 183.1788 24.01988 179.7992 28.13446 64.45215
169 0 TB8742 162.5674 124.4889 187.3836 22.99754 184.1126 26.75049 65.62922
170 0 TB8744 160.4741 122.5939 184.7893 23.95193 181.2449 28.37582 65.10837
171 0 TB8745 158.5020 121.1427 183.3897 24.21397 179.3370 28.44731 64.02206
172 0 TB8746 158.6553 121.2735 183.9315 23.61219 180.2526 27.84734 64.36702
173 0 TB8747 158.9035 121.4250 183.6830 24.03438 180.2482 28.21314 64.59273
174 0 TB8748 160.0395 122.3756 184.6735 23.83354 181.0567 28.05097 64.88185
175 0 TB8749 159.5480 121.8331 184.0166 23.83190 180.2022 28.01274 65.05938
176 0 TB8750 159.2509 121.4413 183.4731 23.82992 180.2052 27.92439 65.05720
177 0 TB8751 159.0554 121.2670 183.2693 23.89857 179.7541 28.28613 64.97931
178 0 TB8752 159.6180 121.7105 184.0884 23.77058 180.3204 27.96352 64.88107
179 0 TB8753 160.6501 122.6756 184.9060 23.68645 181.3622 27.79037 64.97721
180 0 TB8754 158.3935 121.0700 183.2949 23.97613 179.3611 28.30396 64.67557
181 0 TB8755 159.7499 122.0814 184.2878 23.68263 180.7417 27.85090 64.96895
182 0 TB8756 161.5166 123.5152 186.2225 22.99566 182.8928 26.86293 65.71652
183 0 TB8757 158.4479 120.7499 183.0825 24.19463 179.4344 28.47269 64.47850
184 0 TB8758 158.8640 121.2194 183.6026 23.98698 180.0462 28.14443 64.62184
185 0 TB8760 158.9185 121.2187 183.1259 23.91082 179.6199 28.18411 64.81342
186 0 TB8761 158.2187 120.8822 183.6206 23.56965 179.6973 27.84446 64.54418
187 0 TB8762 159.0147 121.2171 183.2381 24.17841 179.7256 28.35612 64.95315
188 0 TB8764 159.1426 121.3354 183.1328 24.07554 179.5995 28.36272 65.06729
189 0 TB8765 157.8653 120.3753 182.0453 24.37025 178.2907 28.87278 64.91043
190 0 TB8766 161.2016 122.8977 184.9533 23.90433 181.5257 28.18323 65.51865
191 0 TB8767 161.4082 123.0689 185.6129 23.63356 181.8940 27.64513 65.42715
192 0 TB8768 159.1710 121.4648 184.0355 23.48592 180.3917 27.68597 64.99363
193 0 TB8769 158.2249 120.7300 183.2338 23.87675 179.5364 28.22760 64.48395
194 0 TB8770 157.7859 120.3451 182.5216 24.40590 178.6750 28.85275 64.31890
195 0 TB8771 159.2449 121.8045 184.3252 23.71766 180.8131 27.86331 64.66681
196 0 TB8772 158.7955 121.0514 183.2116 23.73001 179.8327 27.96819 65.13647
197 0 TB8775 159.8817 121.9821 184.4471 23.94611 180.7013 28.17312 64.63886
198 0 TB8789 159.6645 121.9462 183.9461 23.94094 180.2205 28.14979 64.91329
199 0 TB8790 163.6984 125.4053 188.9950 22.45366 185.5025 26.29087 65.67651
200 0 TB8792 159.4515 121.6056 183.2824 24.36528 179.7757 28.64952 65.10353
201 0 TB8793 158.6636 121.2117 183.4969 23.74622 180.1065 27.80646 64.59141
202 0 TB8794 159.1842 121.3417 183.3870 23.98583 179.5637 28.28177 65.41195
203 0 TB8795 159.1012 121.4236 183.2550 23.94232 179.5698 28.33179 65.10547
204 0 TB8796 159.4197 121.6690 184.3809 23.72985 180.6919 27.92411 64.66995
205 0 TB8797 164.0851 125.5835 188.8891 22.73684 185.6667 26.54078 65.87816
206 0 TB8798 162.4213 124.1107 186.7109 23.26431 183.3783 27.22811 65.58244
207 0 TB8799 159.9178 122.3252 185.0837 23.41737 181.4274 27.66066 64.68654
208 0 TB8800 163.5982 125.2694 188.7913 22.61926 185.3229 26.46476 65.58360
209 0 TB8801 158.9554 121.5512 183.8826 23.81276 180.1478 28.08863 64.57620
210 0 TB8802 158.8635 121.0979 183.2573 24.11535 179.6981 28.28724 64.88690
211 0 TB8803 160.1491 122.1541 184.5552 23.67264 180.7738 27.90006 65.35488
212 0 TB8808 158.7510 120.9893 183.0714 24.30290 179.5124 28.56824 64.78837
213 0 TB8809 158.6629 121.0151 182.8436 23.99520 179.0431 28.34372 65.04519
214 0 TB8810 158.4713 120.8658 182.8303 24.21561 179.2412 28.61688 64.83802
215 0 TB8811 158.7790 121.1479 183.2710 24.25449 179.3486 28.74092 64.90685
216 0 TB8812 159.3819 121.7413 184.2261 23.77598 180.4631 27.97969 64.73008
217 0 TB8813 160.2202 122.5210 185.2137 23.02681 181.6000 27.00590 65.45579
218 0 TB8814 160.4000 122.7684 185.7430 23.61012 181.7227 27.93059 64.50769
219 0 TB8817 164.3214 125.7528 188.8960 22.69877 185.3010 26.63195 65.95279
220 0 TB8818 159.9623 122.1737 184.9553 23.98777 180.6740 28.44482 64.29461
221 0 TB8819 164.1895 125.6884 189.5042 22.05446 186.0577 25.75052 66.19110
222 0 TB8820 162.1130 123.8569 186.6915 23.42951 183.0595 27.42782 65.37109
223 0 TB8821 159.0805 121.3519 183.6571 23.77448 179.9015 28.09023 64.89931
224 0 TB8822 160.5884 122.9335 185.9959 22.69302 182.6763 26.58497 65.32769
225 0 TB8823 162.1947 124.1491 186.9592 22.94702 183.6672 26.93674 65.58316
226 0 TB8824 158.1729 120.7148 183.6098 23.56590 179.8228 27.79391 64.57856
227 0 TB8825 161.3210 123.5915 186.5503 22.90760 182.8496 26.96576 65.23055
228 0 TB8826 158.5046 121.1151 183.6554 23.87549 179.9280 28.23838 64.29771
229 0 TB8827 158.8783 121.2781 183.9279 23.64148 180.3249 27.71124 64.84033
230 0 TB8828 161.2604 123.1657 185.8545 23.51655 182.1003 27.50764 65.49642
231 0 TB8829 158.7600 121.2731 183.3931 23.99968 179.6148 28.34444 64.54324
232 0 TB8830 161.8070 123.6289 186.6206 23.07460 183.0342 27.07681 65.61973
233 0 TB8831 161.2127 123.0695 185.5428 23.30585 181.9786 27.36117 65.45892
234 0 TB8832 164.2570 125.8606 188.6884 22.57162 185.1694 26.45743 66.03561
235 0 TB8839 161.3243 123.2937 186.0492 23.38258 182.3783 27.47002 65.15799
236 0 TB8841 160.8016 122.9092 185.6367 23.33733 182.0610 27.30737 65.16847
237 0 TB8843 161.3197 123.3206 186.0319 23.41956 182.5054 27.49871 65.26721
238 0 TB8844 159.5481 121.9366 184.3832 23.63737 180.9145 27.87934 64.86071
239 0 TB8845 159.0124 121.5352 183.6509 23.91959 180.1195 28.16313 64.66371
240 0 TB8846 160.8008 123.2056 186.1891 23.25151 182.7299 27.33993 64.76713
241 0 TB8847 158.8167 121.0562 183.2588 23.85864 179.2529 28.35099 64.76000
242 0 TB8848 159.5756 121.8116 184.2886 23.88984 180.5019 28.18577 64.84034
243 0 TB8849 160.4436 122.4946 185.2182 23.46200 181.6373 27.56250 65.09428
244 0 TB8850 160.2790 122.4022 185.0135 23.47829 181.3003 27.57531 64.98536
245 0 TB8852 160.2703 122.4821 184.9624 23.26434 181.8272 27.21611 65.08309
246 0 TB8853 159.4309 121.7466 184.4307 23.53538 180.5351 27.58328 64.81684
247 0 TB8854 162.9513 124.4864 187.1458 23.17680 183.8293 27.14185 65.98684
248 0 TB8855 163.8935 125.3102 188.3529 22.81452 185.0300 26.58101 65.96321
249 0 TB8856 160.3827 122.6149 185.7556 23.54266 181.6528 27.74708 64.45583
250 0 TB8857 158.5481 121.1009 183.5705 23.42242 180.0082 27.58799 64.96415
251 0 TB8858 162.1772 124.1990 186.8074 23.16434 183.5396 27.11217 65.63726
252 0 TB8859 163.7779 125.2432 188.4481 22.62832 185.2280 26.55266 66.08440
253 0 TB8860 158.2405 120.9144 183.3232 23.68533 179.6447 28.03970 64.33551
254 0 TB8861 159.5205 121.7023 183.5650 24.12784 179.7549 28.47420 64.88205
255 0 TB8862 158.5723 120.9819 183.2569 24.09007 179.6283 28.39174 64.46453
256 0 TB8863 161.2820 123.2757 185.4214 23.46313 181.9667 27.57375 65.49327
257 0 TB8864 159.0524 121.3104 183.6581 24.06295 179.9523 28.40480 64.89280
258 0 TB8879 164.4974 125.9605 189.1727 22.34525 185.6296 26.14055 66.28169
259 0 TB8880 160.0530 122.3824 185.2069 23.13199 181.4725 27.42103 65.03851
260 0 TB8881 159.8549 122.0170 183.8306 24.38986 180.4990 28.68922 64.85688
261 0 TB8882 164.3607 125.8313 189.2070 22.45571 186.2137 26.04754 66.07162
262 0 TB8883 164.9327 126.5149 190.2363 22.39478 186.6428 26.18859 65.90904
263 0 TB8884 163.0228 124.6918 187.5714 23.21442 184.5166 27.11231 65.73079
264 0 TB8886 160.6455 122.7414 185.0426 23.19193 181.9778 27.07031 65.49190
265 0 TB8887 159.8584 122.0105 183.9879 23.90395 180.6426 27.99657 65.03212
266 0 TB8888 163.7213 125.3785 188.7556 22.66303 185.6395 26.26017 65.70783
267 0 TB8889 163.9777 125.4712 188.6384 22.68819 185.5425 26.46378 66.14049
268 0 TB8890 163.3712 125.0321 188.1899 22.81736 184.7636 26.58633 65.60986
269 0 TB8891 163.8536 125.6327 188.4741 22.78005 185.5866 26.48475 65.78918
270 0 TB8892 163.4710 125.1462 188.2760 23.11214 184.7118 27.01257 65.67817
271 0 TB8899 160.9488 123.1199 185.0536 23.47498 181.8225 27.51533 65.53826
272 0 TB8900 159.9077 121.9352 183.9589 24.26473 180.2521 28.73930 64.91517
273 0 TB8901 160.2192 122.3235 184.5327 23.87228 180.9857 28.16143 65.10299
274 0 TB8902 164.1105 125.9068 189.5683 21.99368 186.6862 25.62175 66.02704
275 0 TB8903 158.7414 120.9745 182.4122 24.39026 178.6947 28.63680 64.99771
276 0 TB8904 158.9666 121.2093 183.2077 23.95962 179.5085 28.07313 65.44766
277 0 TB8905 160.2165 122.4226 185.4955 23.55233 181.4106 27.70207 64.68502
PEL_dist KHV_dist ACB_dist GWD_dist ESN_dist MSL_dist CEU_dist YRI_dist
1 18.15546 169.4924 45.48770 103.35244 171.3722 199.9559 131.4424 147.1639
2 18.06668 167.7712 46.51253 105.06937 173.2636 202.2976 129.9545 148.8648
3 17.65154 170.5250 45.58631 103.07361 170.6094 199.0414 132.4537 146.5428
4 18.73566 167.5581 46.57154 104.86523 172.1543 200.9086 130.3550 148.0503
5 18.00020 168.9929 46.30728 104.27954 171.7769 200.4969 131.3468 147.7636
6 17.98129 169.1242 45.85486 103.67613 171.7378 200.2793 131.1288 147.4924
7 18.37466 168.1960 46.27296 104.52093 172.2437 201.1583 130.4283 148.2062
8 17.53609 174.3139 43.66099 100.02088 167.2547 195.0929 135.4807 143.4116
9 18.30362 168.7204 46.05345 104.01800 171.6723 200.3908 130.7255 147.6534
10 18.34026 168.4484 46.71362 105.04534 171.7546 200.7290 131.0644 147.9287
11 18.25497 169.3984 45.69426 103.74588 171.4867 200.2647 131.4205 147.4422
12 17.99148 169.6330 46.00457 103.72394 170.9186 199.5665 131.8646 147.0237
13 18.60097 168.3723 46.30473 104.53585 171.8296 200.6955 130.7934 147.9440
14 17.94711 169.6895 45.47126 103.14694 171.4136 199.9301 131.2936 147.0741
15 18.68201 168.1929 46.49578 104.73867 171.8733 200.9114 130.9174 148.0270
16 17.68896 174.0198 44.39201 100.56746 166.7713 194.7304 136.1457 143.3347
17 18.07092 169.7920 45.41858 103.06598 171.0511 199.5255 131.4277 146.8829
18 17.75398 172.0001 44.42399 101.67747 169.4639 197.7458 133.4970 145.3638
19 17.77087 172.1098 44.60064 101.72535 169.1615 197.3372 133.5802 145.1443
20 17.50016 173.5049 44.25359 101.05729 168.1710 196.2253 135.1772 144.1594
21 17.85679 171.1608 45.14488 102.40349 169.9209 198.3037 133.0522 145.9704
22 18.12859 168.7674 46.01671 104.25114 172.1715 200.9539 130.8674 148.0358
23 18.13673 169.0263 46.12257 104.23851 171.7282 200.5677 131.2561 147.7247
24 18.51050 169.4026 45.90598 103.75205 170.4877 199.1906 132.0516 146.7550
25 17.59475 173.2309 44.18832 101.02427 168.2504 196.2464 134.5875 144.3057
26 17.99845 169.7898 45.35114 103.11966 171.2796 199.6959 131.6205 146.9263
27 18.97988 167.6581 46.85995 105.16350 171.5214 200.5007 130.7177 148.0556
28 18.51203 168.9243 46.52034 104.34275 170.9035 199.7717 131.7722 147.3777
29 18.32872 168.2698 46.05777 104.15540 172.1860 200.8927 130.3249 147.9540
30 18.46590 169.0635 45.51276 103.50317 171.5281 200.1992 131.0475 147.3999
31 18.67974 167.7115 46.40805 104.76015 172.4515 201.3763 130.3017 148.4516
32 18.40607 167.5658 47.09488 105.31854 172.2362 201.1307 130.5015 148.4317
33 18.24269 168.9814 45.79786 103.79017 171.8398 200.5368 130.8262 147.7660
34 18.20103 168.5069 46.09054 104.18649 172.2482 201.1788 130.5639 148.0265
35 18.24898 168.3857 46.35873 104.48704 171.9577 200.7460 130.8210 147.9249
36 17.89744 170.6520 45.71589 103.11863 170.3515 198.9744 132.5201 146.6178
37 18.33570 168.6160 45.62090 103.76313 172.3143 201.0628 130.2708 148.0347
38 17.40420 175.0524 43.74019 99.71608 166.3040 194.0910 136.5792 142.7628
39 17.87983 170.9411 44.94689 102.30684 170.2280 198.5027 132.6051 145.9876
40 18.12016 168.6429 46.38610 104.32325 171.8115 200.4848 130.9198 147.7400
41 18.01611 171.5600 44.91766 102.15440 169.1815 197.5695 133.6748 145.3426
42 17.95143 168.9715 45.97103 103.75912 171.9466 200.6228 130.8742 147.6993
43 18.07491 168.1432 46.87893 104.90590 172.2494 201.1162 130.6796 148.2685
44 17.34042 173.4818 44.39190 100.99740 168.2779 196.3829 134.7894 144.3381
45 17.49227 171.2888 45.05484 102.29279 170.3145 198.7645 132.8284 146.1490
46 18.52698 169.0105 45.56570 103.60715 171.4214 200.1158 131.1897 147.3417
47 17.92484 170.2551 45.06465 102.77786 171.2535 199.7849 131.6645 147.0032
48 17.87555 169.9270 45.80911 103.72660 171.0966 199.8947 131.8264 147.1259
49 17.90887 173.8964 44.05180 100.47556 167.0458 194.9095 135.7827 143.4169
50 18.09014 170.6847 44.98009 102.38551 170.0944 198.4327 132.4935 145.9217
51 17.35210 173.5408 44.67091 100.75441 167.2847 195.0873 135.3856 143.6002
52 18.65399 167.7672 46.82230 105.32753 172.1802 201.3677 130.5615 148.4329
53 18.58919 168.0505 46.54423 104.84220 171.8744 200.8543 130.6571 148.0587
54 18.08808 168.6384 46.32568 104.41380 172.0555 200.9117 130.5694 148.0900
55 18.63333 167.8214 46.63838 104.82177 171.8997 200.6812 130.6304 147.9670
56 18.27601 168.5749 46.42389 104.63717 171.6593 200.4751 131.0487 147.6990
57 18.75197 167.3376 46.25545 104.83860 172.9412 201.9315 129.6641 148.8288
58 18.01636 168.9345 45.63117 103.67656 172.2500 200.9178 130.4836 147.8230
59 17.34804 173.6975 43.97088 100.40378 168.0772 196.0402 134.8785 144.0143
60 17.67363 173.9166 44.03758 100.57869 167.2929 195.3038 135.8181 143.5899
61 17.72391 174.7376 43.63634 99.79726 166.1965 193.9518 136.4338 142.5580
62 18.15891 170.1399 45.33833 103.05409 170.7435 199.2578 131.9758 146.7665
63 17.52607 171.7756 44.46698 101.68809 170.0801 198.3065 133.1790 145.6234
64 18.64819 168.0422 46.01271 104.34326 172.4022 201.2805 130.2583 148.2477
65 18.38475 168.5861 45.66689 103.88074 172.2673 201.0578 130.5677 147.9660
66 17.19799 175.3561 43.43688 99.37361 166.7195 194.2900 136.6025 142.7207
67 17.14284 175.2086 43.65915 99.70248 166.6085 194.4403 136.4494 142.7722
68 17.76107 172.6879 44.04976 101.12053 168.7147 196.8857 134.2385 144.6484
69 17.79688 169.5616 46.12564 104.10831 171.5253 200.3913 131.5088 147.5507
70 17.89405 172.4524 44.29999 101.19606 168.7409 196.7911 134.0247 144.8016
71 18.19632 170.1318 45.70466 103.20723 170.2817 198.8275 132.5920 146.4103
72 17.89593 170.3594 45.43184 102.85763 170.4786 198.8114 132.4043 146.3638
73 17.62924 172.0019 44.78901 101.63115 169.0288 197.2159 133.6917 144.9974
74 18.08878 170.5308 45.07887 102.37466 170.0930 198.3538 132.2675 146.0247
75 17.91460 173.4891 43.75834 100.30350 167.7693 195.6450 135.0514 143.8264
76 18.21177 170.0860 45.51608 103.02885 170.4118 198.8640 132.0421 146.5373
77 17.98112 170.6486 45.24103 102.74218 170.4599 198.8853 132.6612 146.4478
78 18.45672 167.9255 46.61116 104.92986 172.2567 201.2794 130.5269 148.2330
79 17.72311 173.6221 44.11050 100.77814 167.5562 195.5454 135.4204 143.8244
80 17.87012 171.5134 45.13034 102.35703 169.2956 197.6972 133.4700 145.4361
81 17.74490 173.2052 44.33597 100.94863 167.9956 195.9453 135.2095 144.1089
82 17.76546 172.5461 45.06183 102.00088 168.3632 196.7392 134.7152 144.8464
83 18.35363 169.8664 45.31120 102.88636 170.4994 198.8331 131.8481 146.4490
84 18.28660 168.7474 46.26088 103.95376 171.2497 199.8700 131.0006 147.2960
85 17.80762 174.7635 43.41648 99.47109 166.1600 193.8310 136.4494 142.4734
86 17.34790 174.9867 43.73180 99.87188 166.4275 194.1950 136.3790 142.7726
87 18.16573 169.3186 46.22085 103.71871 170.8345 199.3055 131.9180 146.8967
88 18.06327 170.2264 45.55692 103.10512 170.4143 198.9275 132.2969 146.3420
89 17.71575 171.0963 45.42503 103.00141 170.0835 198.7633 132.9876 146.2162
90 17.46117 171.0736 45.23034 102.37436 170.4220 198.6806 132.6710 146.1527
91 17.63197 173.7576 44.25587 100.81752 167.3501 195.3467 135.3914 143.6939
92 17.97626 170.8101 44.42451 101.85719 170.7355 198.8823 131.8077 146.2521
93 17.78970 173.7062 44.06958 100.30594 167.3752 195.2202 135.2638 143.6496
94 17.36603 174.3361 43.87004 100.30593 167.3472 195.1939 135.8230 143.4980
95 17.67803 171.6912 44.93701 101.91658 169.5085 197.7409 133.3179 145.4388
96 17.79269 171.3961 45.00654 102.25805 169.7538 198.1679 133.1180 145.8070
97 17.77612 174.9192 43.92959 100.21775 165.8728 193.8474 137.1127 142.5744
98 18.38726 168.3291 46.37393 104.55249 172.0638 201.0201 130.7668 148.0856
99 17.73252 174.0630 43.89592 100.32819 167.0588 195.0441 135.7894 143.3572
100 17.57776 174.2209 44.12371 100.43618 166.9810 194.9156 135.8124 143.4111
101 17.38662 174.3924 43.93042 100.17894 167.1034 194.9893 135.8329 143.3050
102 17.80197 175.1364 43.48092 99.61462 166.0191 193.8341 136.9622 142.4378
103 17.83445 172.8635 44.20000 100.87696 168.1168 196.1124 134.5189 144.2668
104 18.50126 169.2275 45.61143 103.35627 171.0301 199.5540 131.1853 147.0446
105 18.19601 169.9034 45.78708 103.49932 170.4863 199.1739 132.2993 146.7001
106 17.89188 172.3291 44.10879 101.03029 169.0635 197.3099 133.5339 145.0397
107 17.47505 175.2643 43.14076 99.16248 166.3617 194.0949 136.4072 142.5249
108 17.58058 173.2554 44.21705 101.07176 168.1952 196.4322 134.6894 144.3555
109 17.66061 173.3242 44.44036 101.08204 167.8712 195.9340 135.1793 144.1866
110 17.76442 169.5044 45.76023 103.80457 172.0220 200.8194 131.2130 147.6532
111 18.42574 169.3873 45.74238 103.55516 170.8652 199.4971 131.7462 146.8990
112 17.61151 171.9010 44.94588 101.89524 169.2371 197.4525 133.4761 145.3318
113 18.20848 170.9556 44.95434 102.24071 169.7416 198.0658 132.8966 145.8563
114 18.20671 170.5978 44.68829 101.92144 170.3682 198.6223 132.2602 146.1340
115 18.01362 170.9958 44.86758 101.92683 170.0229 198.2735 132.8199 145.8977
116 18.46494 169.3015 45.99539 103.87558 170.7505 199.4790 131.8006 146.9677
117 18.19409 170.4672 45.19379 102.72221 170.2402 198.6772 132.5074 146.2928
118 18.02670 169.8086 45.56068 103.15086 171.1092 199.4797 131.8348 146.9430
119 18.33263 170.4236 45.15524 102.82749 170.2784 198.8719 132.5733 146.2437
120 17.74847 174.1501 43.80131 100.02071 167.1732 194.8663 135.8403 143.2599
121 18.28764 170.3990 45.07524 102.84829 170.3879 199.0811 132.1461 146.4610
122 18.19620 169.3763 45.56865 103.38614 171.6179 200.3105 131.0047 147.4939
123 17.86688 170.0362 45.31741 102.82659 171.1235 199.6295 131.6319 146.7997
124 18.04835 171.2981 45.00859 102.18070 169.4490 197.6330 132.8542 145.5360
125 17.98844 169.8487 45.75117 103.49046 170.8684 199.4541 131.7774 146.9019
126 17.12261 175.2680 43.40880 99.62223 166.7891 194.6179 136.4554 142.8901
127 18.15127 169.3015 46.26282 103.99116 170.9271 199.6491 131.9915 147.0602
128 17.55164 174.2226 43.71874 99.92765 167.0557 194.8450 135.3822 143.2153
129 17.92154 171.6580 44.73568 101.96648 169.4297 197.7972 133.1438 145.5428
130 18.40710 169.2259 45.80680 103.63678 171.1447 199.8514 131.5697 147.2352
131 17.57455 172.3824 44.72560 101.66804 168.8872 197.1513 133.7267 145.0374
132 18.16904 169.6590 45.69076 103.37653 170.8335 199.4138 131.7551 146.8400
133 17.92588 173.1961 44.45975 101.19883 167.5440 195.6299 135.0132 143.9950
134 17.69221 171.1941 44.82363 102.33870 170.2970 198.6536 132.7298 146.0838
135 17.10633 175.2496 43.31273 99.57591 167.0357 194.7967 136.1078 142.9338
136 18.29729 169.4383 45.82988 103.55216 170.8082 199.4157 131.8353 146.8472
137 17.38057 174.4592 43.52272 100.10423 167.6210 195.6429 135.2870 143.7301
138 18.25405 168.2165 46.69147 105.01769 172.1678 201.2532 130.7189 148.3174
139 18.09125 171.4281 44.77601 101.65276 169.2682 197.4133 133.2742 145.3683
140 18.05492 170.2842 45.63439 103.23638 170.2721 198.8292 132.3117 146.4508
141 17.78021 172.0460 44.35565 101.51420 169.5181 197.7596 133.1997 145.4079
142 17.44144 175.0951 43.75283 99.76588 166.3778 194.1291 136.6116 142.7570
143 17.54735 173.7779 44.49188 101.17582 167.4873 195.6146 135.6236 143.9008
144 18.48922 167.9068 46.22205 104.80634 172.8047 201.8431 130.2104 148.6015
145 18.07708 169.4496 45.93034 103.69540 171.2989 199.9965 131.3914 147.3989
146 17.49940 174.0216 43.44916 99.93538 168.0283 195.8625 134.5337 144.0141
147 17.57636 174.2368 44.11651 100.27678 166.6721 194.4723 135.8992 143.1457
148 16.99902 174.8030 44.05732 100.16681 166.8777 194.7076 136.2372 143.0007
149 16.67583 174.5970 43.63319 99.92759 168.0337 195.7390 135.2049 143.7096
150 17.46543 173.0361 44.11297 100.95353 168.8397 196.8397 134.2266 144.7758
151 17.84647 170.9194 45.05630 102.42188 170.4554 198.9289 132.5813 146.3575
152 17.72915 171.0588 45.05337 102.58197 170.3596 198.9053 132.3966 146.3486
153 18.48752 169.2218 45.69884 103.38102 170.7513 199.2604 131.5150 146.7432
154 17.66449 174.3472 43.60948 100.08313 167.1531 195.1070 135.6154 143.4338
155 17.91600 171.9673 45.01724 101.90967 168.6818 196.8888 134.1409 145.0489
156 17.70891 173.5203 44.04225 100.45055 167.6454 195.5715 135.0851 143.8405
157 17.53071 174.8652 43.31990 99.79126 167.0113 194.8645 135.9159 143.1275
158 18.67757 170.1931 45.34469 103.01288 169.6523 198.2137 132.8029 145.9884
159 18.17916 169.8073 46.16531 103.77965 170.6432 199.3998 132.2752 146.9521
160 17.74207 170.8122 45.41006 102.91940 170.4185 198.8933 132.8052 146.3461
161 17.97947 170.6093 44.95604 102.49093 170.5270 198.9662 132.1940 146.3974
162 17.96486 170.3800 45.49370 102.95268 170.3975 198.8592 132.4164 146.3648
163 18.23360 168.5059 46.42350 104.43184 171.9156 200.6637 131.2157 147.8421
164 18.31025 169.7779 45.36760 103.13296 171.0747 199.7713 131.8006 147.0135
165 18.23496 169.1771 45.70079 103.63962 171.5109 200.2316 131.3486 147.4155
166 18.17241 171.0319 44.86204 102.00191 169.4981 197.7574 132.8992 145.5032
167 17.98659 170.2756 45.39442 102.91794 170.6889 199.1557 132.1397 146.6134
168 18.32118 168.4048 46.01456 104.23002 172.2614 201.0279 130.5453 147.9806
169 17.47389 173.0302 44.48076 101.23754 168.2276 196.3239 134.5754 144.3953
170 18.74089 169.8918 45.51713 103.01178 169.8723 198.3847 132.2789 146.1888
171 18.08161 168.3652 46.04200 103.89523 172.4070 200.8527 130.4833 147.8977
172 18.19092 169.0364 45.42735 103.43854 172.0283 200.6191 130.7375 147.6567
173 18.18074 168.9716 45.86158 103.78566 171.5847 200.1923 130.9496 147.3813
174 18.01978 170.0176 45.51477 102.89653 170.5715 198.8997 132.0923 146.4472
175 18.08150 169.3446 45.98638 103.63355 171.2156 199.8559 131.6162 147.2319
176 17.98116 169.1191 46.10796 104.22954 171.8382 200.5976 131.4568 147.6181
177 18.50154 168.6081 46.14656 104.28678 171.8202 200.6377 131.1170 147.7910
178 18.29335 169.2794 45.81030 103.82557 171.4076 200.1739 131.6129 147.3643
179 18.37272 170.0514 45.50860 103.21040 170.3896 198.9730 132.4276 146.5154
180 18.20172 168.4112 46.32666 104.30669 172.0351 200.7656 130.4093 148.0307
181 18.01484 169.6779 45.79185 103.47364 171.0479 199.5809 131.7871 146.9975
182 17.70665 171.8594 44.94693 102.16544 169.3170 197.7618 133.6077 145.5407
183 18.65278 168.1244 45.92517 104.28741 172.2535 201.1667 130.4618 148.0363
184 18.15117 168.8970 45.99332 104.11504 171.8319 200.5803 130.9411 147.6313
185 18.52252 168.3072 46.27127 104.62466 172.0898 200.9947 130.8249 148.1006
186 18.01843 168.8354 45.83141 103.84015 172.3417 201.0573 130.4293 148.0660
187 18.36402 168.5479 46.33902 104.55456 171.6970 200.6195 131.0241 147.7479
188 18.39616 168.4896 46.37801 104.50627 171.7599 200.6078 131.1655 147.8014
189 18.76652 167.1237 46.97920 105.52945 172.5906 201.7059 129.7956 148.8298
190 18.65907 170.2333 45.76656 103.41610 169.5470 198.2518 132.9778 146.0557
191 17.90205 171.0672 45.34653 102.80151 169.7646 198.2693 133.4648 145.8371
192 18.11035 169.4139 45.63057 103.62229 171.6545 200.4117 131.3729 147.5132
193 18.31405 168.4374 46.02137 104.26807 172.3547 201.1549 130.3506 148.1115
194 18.84378 167.3805 46.29006 104.66593 172.5866 201.5298 129.7112 148.4790
195 17.92569 169.6761 45.63088 103.33863 171.2283 199.7093 131.3377 147.0311
196 18.05548 168.8944 46.23412 104.37533 172.0801 200.9328 131.0974 147.9240
197 18.46367 169.4673 45.33096 103.19924 171.0505 199.6201 131.8236 146.8566
198 18.24423 169.1075 46.07482 103.94876 171.1988 199.8545 131.5437 147.2845
199 17.63311 174.4685 43.46025 99.73415 167.0934 194.9567 135.6510 143.2922
200 18.44676 168.6048 46.46855 104.45559 171.2216 200.0059 131.3896 147.3887
201 17.97538 168.9390 45.86885 103.86293 172.1820 200.8445 130.8212 147.8040
202 18.55364 168.6358 46.40079 104.44402 171.3753 200.4134 131.2030 147.7564
203 18.32069 168.5520 46.45861 104.56213 171.6948 200.4880 131.1008 147.8399
204 18.18135 169.6250 45.37582 103.18267 171.2985 199.8904 131.5479 147.0326
205 17.57125 174.5918 43.60463 99.85083 166.7713 194.5936 136.0963 142.9522
206 17.76825 172.3390 44.91870 101.89334 168.5863 196.7967 134.3814 144.7822
207 18.07892 170.2830 45.08052 102.61865 170.7205 199.0756 131.9516 146.5325
208 17.55895 174.2342 43.47321 100.02398 167.3612 195.2166 135.5766 143.4232
209 18.25227 168.9580 45.70770 103.58925 171.6323 200.2019 130.9357 147.4825
210 18.31731 168.5944 46.23174 104.37389 171.8217 200.7240 130.9428 147.7798
211 18.24540 169.8617 45.86439 103.65752 170.5920 199.3669 132.1620 146.8696
212 18.57675 168.2470 46.32252 104.62606 171.8197 200.7769 130.7169 147.8647
213 18.20434 168.1957 46.67127 104.84850 172.2384 201.1073 130.7536 148.2729
214 18.64768 168.0048 46.36702 104.61561 171.9994 200.9184 130.4664 148.0641
215 18.58944 168.3967 46.31439 104.21994 171.5267 200.3058 130.8023 147.6576
216 18.08634 169.4702 45.63315 103.35805 171.3192 199.8716 131.4681 147.1353
217 17.44072 170.9175 45.41211 102.81245 170.6488 199.1396 132.5245 146.6085
218 18.21157 170.6627 44.83121 102.01134 170.0249 198.1974 132.3051 145.9016
219 17.79958 174.2785 43.79518 100.19376 166.7549 194.6659 136.1194 143.1901
220 18.80290 169.4774 45.18483 102.85089 170.6519 199.1262 131.6568 146.6446
221 17.21565 175.2907 43.44142 99.72020 166.8372 194.7765 136.3203 143.1083
222 17.98303 172.0057 44.86856 101.94712 168.6641 196.9796 133.9830 144.9330
223 18.55153 168.7067 45.88760 104.13805 171.7150 200.6091 131.0351 147.7907
224 17.56019 171.6550 44.80905 102.10277 170.1173 198.5371 132.8005 146.0769
225 17.77849 172.5613 44.65828 101.49422 168.5113 196.6765 134.1716 144.7549
226 18.13833 168.8642 45.48029 103.61438 172.4417 201.2307 130.4939 148.0257
227 17.61979 171.9491 44.78730 101.75993 169.3878 197.5450 133.3435 145.4641
228 18.52919 168.6344 45.51301 103.46775 171.9746 200.5887 130.5171 147.6736
229 17.93941 169.4192 45.69893 103.48126 171.7465 200.4628 131.1560 147.4848
230 17.76483 171.3552 45.26979 102.41556 169.4787 197.9027 133.3714 145.6373
231 18.41659 168.4332 46.16184 104.21446 171.9017 200.6074 130.6536 147.8577
232 17.91029 172.0816 44.51586 101.63857 169.0272 197.3783 133.9227 145.1553
233 17.84770 171.1561 45.25307 102.51517 169.9764 198.3855 133.3456 145.9662
234 17.61160 174.2073 44.25581 100.52074 166.8608 194.7548 135.9892 143.4292
235 18.16216 171.2209 44.86876 102.19961 169.4802 197.8748 133.2039 145.6282
236 17.79841 171.0825 45.06416 102.37334 170.0341 198.4350 132.8896 145.9877
237 17.86619 171.4954 44.88867 102.01814 169.4054 197.6535 133.3948 145.4043
238 18.18712 169.6660 45.59833 103.47104 171.0251 199.6122 131.5600 147.0007
239 18.22400 168.8930 46.01827 103.92857 171.6036 200.2186 130.9809 147.5117
240 18.02013 171.4199 44.45248 101.51452 169.6238 197.7504 132.7778 145.4771
241 18.82361 168.1245 46.16636 104.54319 172.1178 201.0913 130.6788 148.2430
242 18.20807 169.5085 45.62098 103.39434 171.0727 199.6352 131.6634 146.9568
243 18.05682 170.5687 45.31183 102.94491 170.3253 198.9010 132.4748 146.3701
244 18.03030 170.2768 45.41714 103.09175 170.6470 199.2150 132.2719 146.6431
245 17.92479 170.4375 45.29003 103.27135 170.8191 199.4169 132.2896 146.7335
246 17.68676 169.8525 45.61023 103.24116 171.5512 200.0945 131.6773 147.2239
247 17.98933 172.7361 44.66740 101.53448 167.8970 196.1881 134.8882 144.3495
248 17.57701 174.0706 44.16389 100.59292 167.1829 195.2089 135.8448 143.5013
249 18.33646 170.5745 44.63293 101.93052 170.2667 198.5832 132.2819 146.0817
250 18.14264 168.9073 45.91173 104.09495 172.1023 201.0091 130.6935 148.0745
251 17.46553 172.5486 44.81705 101.60302 168.5535 196.5890 134.2639 144.6740
252 17.45248 174.3490 43.89922 100.31643 167.2222 195.1207 135.9436 143.3779
253 18.20516 168.5371 45.94961 104.02808 172.4984 201.1291 130.3375 148.1399
254 18.58815 168.5809 46.28272 104.33054 171.3555 200.1372 131.3060 147.5461
255 18.62670 168.2128 45.97611 104.40149 172.1016 200.9783 130.4646 148.0073
256 18.02579 170.8774 45.63720 102.98893 169.6019 198.0607 133.1485 145.9556
257 18.29936 168.9586 45.92357 103.89571 171.4915 200.2011 131.2130 147.3831
258 17.42448 174.8287 43.88937 100.09467 166.6313 194.5470 136.4178 143.1360
259 18.04608 170.5062 45.17576 102.71436 170.6239 199.0849 132.1480 146.6180
260 18.56915 169.0610 45.98794 103.82546 170.7307 199.3092 131.7099 146.7829
261 17.31110 175.1183 43.57741 99.81303 166.6456 194.5060 136.4044 142.8510
262 17.33998 175.7987 43.08172 98.71918 165.8004 193.2975 136.8818 142.0535
263 17.59307 173.4345 44.31166 100.81705 167.6567 195.5945 135.1463 143.7334
264 17.82238 170.8122 45.46780 103.02242 170.4092 199.0100 132.7604 146.4547
265 18.09698 169.4664 46.11857 103.99261 171.0790 199.7468 131.8340 147.0977
266 17.38160 174.5432 43.70937 99.95126 167.1348 195.0080 135.7497 143.2261
267 17.53366 174.4885 43.86432 100.19905 166.8457 194.7951 136.0372 143.1554
268 17.55324 173.7133 43.94846 100.54004 167.6965 195.6653 135.3228 143.8071
269 17.33179 174.3296 43.89774 100.23442 167.1446 194.8554 135.8288 143.2186
270 17.65960 173.7946 44.07795 100.30217 167.1430 195.0119 135.4321 143.3956
271 17.79336 170.7814 45.80962 103.02634 169.8945 198.2775 132.9538 146.1114
272 18.68142 169.0788 45.91945 103.71400 170.7564 199.3564 131.8095 146.8410
273 18.26576 169.8607 45.80389 103.48495 170.4354 198.9895 132.1758 146.5742
274 16.74830 175.7965 43.20526 99.17108 166.8628 194.3914 136.4477 142.7037
275 18.39517 167.6832 46.94744 105.22885 172.3228 201.2897 130.6844 148.3991
276 18.08635 168.7376 46.64776 104.64948 171.6673 200.6896 131.1285 147.9195
277 18.08184 170.5165 44.98355 102.39429 170.4372 198.8600 132.2298 146.3082
CHB_dist JPT_dist LWK_dist ASW_dist MXL_dist TSI_dist
1 146.1137 231.4965 71.27395 30.97249 31.30307 176.1964
2 144.3354 229.5570 72.50585 31.43760 31.41642 174.4418
3 146.7793 232.0441 70.99379 30.27997 31.26070 177.2242
4 144.7433 229.7074 71.99391 32.45143 31.70654 175.0265
5 145.4555 230.7017 71.78960 31.12235 31.29717 176.3429
6 145.6094 230.8022 71.40206 31.10179 31.25604 175.7355
7 144.9295 230.1164 72.13188 31.35879 31.24480 175.1939
8 150.4840 236.7648 69.03835 29.13645 30.80914 180.8733
9 145.3898 230.7927 71.61039 31.23232 31.04814 175.5017
10 145.2594 230.5232 72.22483 31.37805 31.42636 176.0990
11 146.0073 231.4142 71.67865 30.86258 31.28717 176.4121
12 146.1730 231.3887 71.47569 30.79691 31.23480 176.7264
13 145.2705 230.7120 72.00723 31.66198 31.23362 175.9727
14 146.1262 231.6878 71.19626 31.07000 31.04458 175.8645
15 145.0429 229.8465 72.40529 31.85227 31.78270 175.8741
16 150.3024 235.8214 69.49933 29.77922 31.37853 181.7354
17 146.2649 231.6403 71.00234 30.68458 31.10227 176.0225
18 148.3441 234.3953 70.10458 29.77028 31.08094 178.6315
19 148.4826 234.5538 70.02168 29.95391 30.93523 178.7465
20 149.7794 236.1074 69.61288 29.71727 31.16762 180.6382
21 147.5086 233.0447 70.73240 30.43154 31.17402 178.1801
22 145.3383 230.7685 71.81253 30.89772 31.19110 175.8001
23 145.6094 230.9729 71.88522 30.99059 31.27623 176.2366
24 146.4265 231.7196 71.39576 31.25718 31.49548 177.1625
25 149.5214 235.9676 69.42997 29.47417 30.80213 180.0107
26 146.3872 231.8796 71.01169 30.96928 31.24462 176.2850
27 144.9036 229.8778 72.28313 31.84984 31.42607 175.9626
28 145.7260 230.5807 72.08544 31.53050 31.47976 177.0026
29 144.9606 230.1522 71.64257 31.46470 31.33263 174.8474
30 145.8372 231.1384 71.44079 31.15781 31.32311 175.8383
31 144.5918 229.5141 72.24718 31.81192 31.58267 175.1925
32 144.4335 229.2765 72.41007 31.94099 31.41684 175.4034
33 145.4574 230.7608 71.56024 30.79825 31.12712 175.6554
34 145.0343 229.7540 72.21271 31.23865 31.56905 174.8252
35 145.1157 230.0359 71.96549 31.31768 31.45608 175.4679
36 146.8404 232.3824 71.32322 30.45876 30.93270 177.8290
37 145.1834 230.6248 71.64010 31.05797 31.07321 174.9413
38 151.1024 237.0585 68.87671 28.84201 31.03827 182.1298
39 147.3929 233.0496 70.51004 30.66278 31.19286 177.4289
40 145.2733 230.3936 71.82459 31.51201 31.23202 175.6064
41 148.1101 233.5222 70.44799 30.21645 31.54840 178.7608
42 145.3174 230.4907 71.64555 31.21861 31.22099 175.4036
43 144.6117 229.3885 72.32533 31.64858 31.43355 175.3549
44 149.4139 235.4773 69.81264 29.54214 30.94754 180.0371
45 147.3225 232.5472 70.91557 29.89186 31.23482 177.3956
46 145.9051 231.1927 71.45805 31.22016 31.42854 176.0377
47 146.5712 232.1852 71.13614 30.31702 30.95056 176.4801
48 146.3332 231.9186 71.55918 30.29446 31.10346 176.7507
49 150.4212 236.6483 69.19019 29.99387 31.10202 181.6289
50 147.2934 232.6824 70.54581 30.74695 31.43321 177.1459
51 149.6669 235.0443 69.36883 29.76243 31.18713 180.3456
52 144.6646 229.5510 72.66557 31.59322 31.65324 175.5454
53 145.0018 230.0970 72.22941 31.57167 31.40841 175.5803
54 145.1094 230.4738 72.01896 30.86137 30.87111 175.3683
55 144.8414 229.8154 71.95918 32.12947 31.61510 175.4824
56 145.3826 230.6664 71.81163 31.24815 31.43077 175.8884
57 144.3120 229.5618 72.28249 31.68248 31.31115 174.5162
58 145.3782 230.7701 71.56123 30.93386 31.08778 174.8902
59 149.5967 235.4145 69.40858 29.34821 31.15152 179.8199
60 150.2809 236.1550 69.42820 29.47407 31.37303 181.3469
61 151.1975 237.3186 68.68076 29.40573 31.23430 181.9421
62 146.6710 232.3851 70.95349 30.54101 31.04935 177.1456
63 147.9711 233.6961 70.28509 30.12389 31.29269 177.8338
64 144.8667 229.9181 72.03513 31.60638 31.52266 174.9895
65 145.3040 230.6319 71.76398 31.29836 31.36452 175.2643
66 151.2574 237.5347 68.59072 29.28725 31.06136 182.1022
67 151.1075 237.2730 68.92880 28.89772 31.05771 181.7175
68 149.0957 235.0212 69.70195 29.45350 31.33534 179.2884
69 145.8611 231.3908 71.83974 30.44879 31.04649 176.4470
70 148.8415 234.7285 69.76089 29.97668 31.07158 179.3225
71 146.7946 231.8005 71.26302 31.18785 31.62335 177.5590
72 146.8920 232.2192 70.84747 30.87016 31.27107 177.2054
73 148.2797 233.7466 70.14012 30.20358 31.27731 178.4024
74 147.1211 232.6517 70.42629 30.70026 31.09256 177.0321
75 149.9387 236.1737 69.17468 30.06475 31.11357 180.5839
76 146.6940 232.1933 70.94705 30.83400 31.03208 177.0950
77 147.0729 232.5622 70.89562 30.54366 31.27466 177.8395
78 144.7028 229.3801 72.42366 31.68896 31.78687 175.0720
79 150.0424 236.1262 69.40267 29.52256 31.17112 181.0056
80 147.9731 233.5592 70.54351 30.28005 31.30848 178.5159
81 149.5759 235.3053 69.60422 30.21243 31.46345 180.6536
82 148.8516 234.2251 70.51629 29.71129 31.39650 180.1388
83 146.7040 232.3811 70.63604 31.15461 31.10565 176.8173
84 145.4770 230.3102 71.60670 31.48967 31.31048 175.4139
85 151.2589 237.2715 68.54734 29.54696 31.28571 181.9263
86 151.1403 237.6571 68.68781 28.84142 30.78996 181.9927
87 145.9718 230.7415 71.42075 31.73639 31.58709 176.6764
88 146.8574 232.3189 71.05709 31.13713 31.37181 177.1004
89 147.4327 233.1212 71.16877 29.91364 31.14366 178.0675
90 147.1611 232.6090 70.68361 30.49313 31.08937 177.3316
91 150.0740 236.3612 69.32664 29.43779 30.94207 180.9807
92 147.2184 233.2821 70.18471 30.48662 30.75347 176.5476
93 149.9522 236.0354 69.21866 30.07433 30.94293 180.8492
94 150.4659 236.7846 69.06332 29.13020 30.98068 181.3887
95 147.9167 233.5341 70.36876 30.45081 31.12351 178.2226
96 147.6917 233.2808 70.64529 30.08952 31.13579 178.1434
97 151.4402 237.4690 69.07386 29.14580 31.43258 183.0590
98 145.0101 230.0321 72.21344 31.52187 31.49389 175.6121
99 150.4171 236.3418 69.27009 29.50289 31.32037 181.2342
100 150.4113 236.4521 69.30844 29.30040 30.96005 181.4105
101 150.4839 236.6191 69.16115 29.34637 31.00876 181.2199
102 151.5667 237.7006 68.82074 29.50583 31.40494 182.7881
103 149.2856 235.1052 69.51024 29.82513 31.14050 179.7323
104 146.0489 231.2981 71.23194 31.23224 31.12651 175.9396
105 146.6090 231.7322 71.44997 30.76712 31.43834 177.2739
106 148.6090 234.4827 70.04851 29.79094 30.94057 178.5322
107 151.4131 237.6910 68.58946 28.93256 31.00040 181.7515
108 149.4899 235.5380 69.85531 29.17206 31.02879 179.8955
109 149.5993 235.3047 69.80328 29.63143 31.21858 180.6490
110 145.7409 231.2211 71.72454 30.68129 31.28204 175.8285
111 146.2043 231.2334 71.37037 31.26505 31.62091 176.5441
112 148.0867 233.5886 70.29501 29.79627 31.00265 178.3533
113 147.5393 233.0644 70.50596 30.58562 31.23462 178.0828
114 147.1605 232.6553 70.48852 31.01306 31.20089 177.0826
115 147.3604 232.4263 70.58809 30.78394 31.44937 177.5574
116 146.1857 231.3759 71.54285 31.26568 31.47381 176.8286
117 147.1258 232.5579 70.86301 30.67833 31.27486 177.5754
118 146.3012 231.6321 71.02949 31.07841 31.23828 176.7384
119 147.1515 232.4323 71.06153 30.91932 31.70560 177.5024
120 150.5307 236.6565 68.98578 30.20518 31.20361 181.4149
121 147.0919 232.8465 71.07963 30.30262 31.10327 177.1702
122 145.7198 230.8827 71.51995 30.69306 31.18009 175.6521
123 146.3398 231.5577 71.12126 30.82834 31.27968 176.0256
124 147.7414 233.6105 70.10550 30.34360 30.94481 177.9387
125 146.3576 231.8015 71.22189 30.51298 31.08587 176.6123
126 151.2126 237.4798 68.86183 28.46505 31.02853 181.7755
127 146.0088 230.8221 71.78945 31.42296 31.65531 176.7793
128 150.4303 236.6113 68.88822 29.27297 30.82169 180.5345
129 148.0031 233.9024 70.31825 29.82703 30.94839 178.2877
130 145.9476 230.7160 71.66504 31.03826 31.57318 176.3117
131 148.5212 234.5986 70.05122 29.47458 30.76658 178.8521
132 146.2949 231.5374 71.24052 30.95836 31.30814 176.5242
133 149.7383 235.9888 69.56806 29.75200 30.93677 180.7117
134 147.5377 233.4229 70.48876 29.90275 31.05328 177.6519
135 151.1488 237.6498 68.75650 28.65603 30.90464 181.3151
136 146.2317 231.2967 71.37770 31.32087 31.52792 176.6157
137 150.4508 237.1369 69.21786 28.49930 30.55329 180.7747
138 144.8647 229.9194 72.49002 31.18278 31.36232 175.6071
139 147.9490 233.2135 70.31544 30.69121 31.18745 178.2268
140 146.8588 232.3711 71.04889 30.45423 31.11098 177.3246
141 148.2691 234.3105 70.11244 29.74702 30.85545 178.2570
142 151.1930 237.2767 68.93569 29.22054 31.03461 182.2719
143 150.0607 236.2695 69.70846 29.37294 31.04292 181.3645
144 144.7162 229.9161 72.40182 31.48606 31.51375 175.0059
145 145.8653 231.3278 71.55593 30.78852 30.93512 176.4297
146 150.0262 236.8409 69.02869 28.83406 30.14933 179.9333
147 150.5046 236.3964 69.00977 29.17298 30.97788 181.3214
148 150.6742 236.7636 68.96829 29.01824 31.17375 181.3581
149 150.2551 236.6730 68.94571 28.38570 30.51021 179.9894
150 149.1372 235.4568 69.61501 29.34582 30.68246 179.5797
151 147.1504 232.3443 71.04449 30.27328 31.28058 177.3527
152 147.2494 233.2214 70.78526 29.67508 30.77505 177.4145
153 146.1851 231.4711 71.07696 31.59462 31.40348 176.2535
154 150.5788 236.9259 69.11610 28.94806 30.87413 181.2047
155 148.4427 234.0616 70.22903 30.29626 31.15842 179.6176
156 149.7578 235.4380 69.38926 29.64420 31.19771 180.2941
157 151.0860 237.8022 68.86488 28.81520 30.77534 181.5336
158 147.3598 232.6670 70.94460 31.01431 31.61335 178.0269
159 146.2914 231.3320 71.77219 31.16314 31.43295 177.4618
160 147.0774 232.4682 70.92708 30.36695 31.44564 177.7540
161 147.0644 232.7861 70.72956 30.31967 31.01453 177.1028
162 146.9114 232.2167 71.05761 30.88238 31.29698 177.2343
163 145.2125 229.9496 72.05492 31.74143 31.72048 175.9517
164 146.3182 231.3317 71.44958 30.80300 31.58028 176.5751
165 145.8285 230.7811 71.64415 30.90590 31.50229 175.9800
166 147.6739 233.2018 70.27497 30.81723 31.30419 177.7896
167 146.7011 232.1229 71.01984 30.75656 31.19129 177.0496
168 145.1680 230.6871 71.75325 31.59394 31.29250 175.3103
169 149.2721 235.3654 69.68294 29.35031 30.89016 179.7798
170 146.9355 232.1814 71.04956 31.51328 31.38571 177.4118
171 144.8920 229.8354 71.41372 31.83115 31.53246 174.7883
172 145.6249 231.1451 71.33776 31.03650 31.11706 175.4096
173 145.6638 231.0422 71.39335 31.25558 31.21211 175.5307
174 146.6036 231.7008 70.89765 31.15841 31.35656 176.7077
175 145.8487 230.7475 71.61572 31.16604 31.40044 176.3301
176 145.7114 230.9308 71.83945 31.14396 31.55885 176.2004
177 145.4444 230.4736 72.04555 31.70582 31.60807 176.0559
178 145.9550 231.1924 71.65036 31.14441 31.56559 176.5922
179 146.8633 232.4950 71.08140 31.15531 31.39217 177.7591
180 144.9183 229.9745 71.81860 31.05535 31.06906 175.0060
181 146.2120 231.5210 71.29006 31.00355 31.22663 176.6485
182 148.1825 234.0217 70.58664 29.61771 30.93868 178.8547
183 145.0981 230.2454 72.00226 31.74648 31.65934 175.1334
184 145.5144 230.8866 71.63789 31.13927 31.35512 175.6083
185 145.1906 230.5611 72.08018 31.56563 31.41637 175.9530
186 145.1314 230.3646 71.73905 30.79380 31.02976 174.9708
187 145.3637 230.5072 72.02108 31.33902 31.51524 175.8263
188 145.3260 230.2460 72.10294 31.64317 31.62862 176.0323
189 144.1050 228.9164 72.70205 31.70991 31.37903 174.5999
190 147.2751 232.6035 71.29533 31.27938 31.62150 178.4464
191 147.5944 232.7539 70.89948 30.44110 31.79438 178.4286
192 145.9190 231.1820 71.69126 30.74575 31.31115 176.1809
193 145.0393 230.3187 71.88208 31.31376 31.27741 175.0054
194 144.3925 229.3526 72.22028 32.06547 31.48129 174.2941
195 146.1194 231.6630 71.06718 30.72388 30.97981 175.9656
196 145.3857 230.4410 72.14422 31.18934 31.47409 175.8159
197 146.3930 231.5911 71.18028 31.39498 31.75196 176.6247
198 145.8359 231.0298 71.51693 31.19607 31.37218 176.4935
199 150.6325 236.9634 68.96969 29.18053 30.85101 181.1749
200 145.5534 230.4060 71.90007 31.69800 31.65815 176.1777
201 145.4051 230.8561 71.62648 31.25065 31.18419 175.4496
202 145.4043 230.1862 72.32174 31.15082 31.45314 176.1432
203 145.2936 230.2395 72.01455 31.28397 31.40469 176.0153
204 146.2198 231.4938 71.27557 31.08634 31.46021 176.2077
205 150.8924 237.0089 68.94127 29.39599 31.17462 181.4745
206 148.7711 234.5084 70.14444 30.22998 31.33128 179.7009
207 146.8062 232.3960 70.73174 30.73027 31.08422 176.8122
208 150.5443 236.8152 68.91464 28.93556 31.07258 180.9142
209 145.6411 230.9437 71.35173 31.11852 31.14336 175.6360
210 145.3156 230.3976 72.03016 31.41268 31.51604 175.6477
211 146.4689 231.6309 71.56846 30.62623 31.40335 177.2343
212 145.1869 230.3261 72.06766 31.56198 31.54669 175.4990
213 144.7933 229.5350 72.31227 31.40169 31.49770 175.4977
214 144.9517 229.9287 72.17877 31.72325 31.44371 175.2240
215 145.1963 229.8242 71.96756 31.63884 31.51272 175.3862
216 146.0044 231.1641 71.31188 31.07442 31.35236 176.1085
217 146.9390 232.3404 71.10150 29.76079 30.93826 177.3413
218 147.2251 232.6359 70.27116 30.83901 31.19492 177.0579
219 150.7238 236.7518 69.08948 29.28823 31.23450 181.8248
220 146.4409 231.6733 70.87446 31.50287 31.58936 176.6084
221 151.1397 237.5636 69.02366 28.17168 30.80047 181.9090
222 148.5303 234.2528 70.18456 30.13961 31.31375 179.2440
223 145.5726 230.8445 71.89417 31.06237 31.38790 176.0726
224 147.7001 233.8411 70.62203 29.55134 30.54072 177.9483
225 148.8815 234.8891 70.00059 29.93369 30.86420 179.5724
226 145.3179 230.4962 71.80403 30.83205 31.27566 174.9215
227 148.0809 233.9317 70.13235 29.70384 30.73266 178.4767
228 145.4271 230.7184 71.47373 31.65054 31.26077 175.1507
229 145.7313 230.9444 71.67065 30.97602 31.22632 175.6938
230 147.6849 232.8648 70.73437 30.13909 31.39991 178.2060
231 145.1674 230.4122 71.71754 31.54455 31.24376 175.5070
232 148.5505 234.0780 70.36738 29.80596 31.33480 179.0178
233 147.5283 232.7496 70.93079 30.69667 31.56124 178.3961
234 150.4201 236.5962 69.22926 29.36230 30.86250 181.9008
235 147.8658 233.5400 70.48108 30.38658 31.26402 178.4274
236 147.4454 232.9887 70.69101 30.32306 31.19428 177.8163
237 147.9857 233.4159 70.36763 30.37526 31.33150 178.2871
238 146.3218 231.9304 71.19076 30.72164 31.07095 176.4936
239 145.5565 230.9277 71.49312 31.34626 31.14643 175.7758
240 147.9167 233.8596 70.00930 30.47537 30.84090 177.7127
241 145.0575 230.1500 72.23506 31.59336 31.55114 175.8616
242 146.1854 231.2004 71.29412 31.03007 31.53584 176.2777
243 147.0807 232.6919 70.99630 30.46839 31.24186 177.5421
244 146.7695 232.2969 71.07824 30.57991 31.26999 177.3220
245 147.0288 233.1676 70.97957 30.08944 31.01816 177.5226
246 146.0168 231.1055 71.36203 30.68948 31.37310 176.1945
247 149.3346 235.0175 70.13034 29.97857 31.35589 180.3637
248 150.3085 236.3327 69.44733 29.58511 31.23022 181.4023
249 147.1829 232.6256 70.46888 30.94906 31.35972 177.1171
250 145.3758 230.9118 71.96468 30.57106 30.88875 175.6244
251 148.8299 234.5437 69.86544 29.67450 31.00708 179.2645
252 150.5501 236.4164 69.31334 29.28537 31.29453 181.2650
253 145.0187 230.4060 71.70331 31.51236 31.12341 175.0573
254 145.5215 230.5736 71.82091 31.65631 31.56567 176.3850
255 145.1945 230.6526 71.81739 31.39685 31.38247 175.3482
256 147.4368 232.9986 70.88322 30.59715 31.14824 178.5249
257 145.6959 230.5464 71.68932 31.21140 31.60921 175.7123
258 150.8998 237.0277 69.14417 28.76500 30.95110 182.1856
259 146.8890 232.4396 70.97241 30.38695 30.99056 177.2031
260 146.1773 231.3169 71.33591 31.85595 31.65671 176.5251
261 151.2086 237.7344 68.88586 28.88130 30.91665 181.9604
262 151.7924 238.0053 68.16929 28.80827 30.88445 182.2880
263 149.8126 235.5715 69.50557 30.00360 31.30398 180.1713
264 147.1924 233.0253 71.17530 30.44258 31.05616 178.0123
265 146.1082 231.5212 71.51097 31.24247 31.38460 176.8358
266 150.6071 237.0463 69.01479 29.34790 30.88143 181.1309
267 150.7524 236.8752 69.22361 29.21401 31.07704 181.4894
268 150.0048 236.1119 69.30501 29.41053 31.14716 180.6821
269 150.6092 236.9809 68.83938 29.29393 30.91468 181.1712
270 150.0884 235.7963 69.21772 29.76902 31.20301 180.5774
271 147.1798 232.6348 70.96648 30.78726 31.04548 178.1075
272 146.1435 230.9344 71.48761 31.93794 31.87296 176.6449
273 146.6042 231.8539 71.20583 31.07797 31.41676 177.1516
274 151.4681 238.0402 68.44727 28.28773 30.60826 181.6279
275 144.5230 229.1695 72.51925 31.96292 31.75931 175.4629
276 145.1915 230.0041 72.37791 30.87626 31.32535 175.8606
277 146.9506 232.2890 70.71780 30.54906 31.33282 176.9661`summarise()` regrouping output by 'FID' (override with `.groups` argument)PLINK v1.90b6.18 64-bit (16 Jun 2020) www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang GNU General Public License v3
Logging to genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.ancestry.log.
Options in effect:
--bfile genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter
--keep genotype_qc/raw_data_assigned_ancestry.clst
--make-bed
--out genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.ancestry
192030 MB RAM detected; reserving 96015 MB for main workspace.
1826278 variants loaded from .bim file.
277 people (150 males, 127 females) loaded from .fam.
--keep: 277 people remaining.
Using 1 thread (no multithreaded calculations invoked).
Before main variant filters, 277 founders and 0 nonfounders present.
Calculating allele frequencies... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99% done.
Total genotyping rate is 0.997271.
1826278 variants and 277 people pass filters and QC.
Note: No phenotypes present.
--make-bed to
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.ancestry.bed
+
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.ancestry.bim
+
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.ancestry.fam
... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99%done.[Mon Apr 11 16:28:27 2022]
Finished job 0.
1 of 1 steps (100%) done
Complete log: /scratch/st-dennisjk-1/wcasazza/delahaye_QC/placenta_regulatory_landscape/RootStudyConsentSet_phs001717.PlacentalRegulation.v1.p1.c1.HMB-IRB-PUB-COL-MDS/.snakemake/log/2022-04-11T162821.601075.snakemake.logassigned_ancestry <- fread(paste0(BASE_DIR,"raw_data_assigned_ancestry.clst"))
table(assigned_ancestry$V3)
PEL
277 assigned_ancestryThe above looks suspicious, let’s see if GRAF-pop (another method for inferring ancestry based on a different model with selected “ancestry-informative markers”) agrees:
assign_ancestry <- function(graf_pop){
ancestry <- rep("",nrow(graf_pop))
afr_idx <- graf_pop$`F(%)` >= 95
eur_idx <- graf_pop$`E(%)` >= 87
eas_idx <- graf_pop$`A(%)` >= 95
afr_am_idx <- (graf_pop$`F(%)` < 95) & (graf_pop$`F(%)` >= 40) & (graf_pop$`A(%)` < 13)
lat_am_1_idx <- (graf_pop$`F(%)` < 40) &( graf_pop$`E(%)` < 87) & (graf_pop$`A(%)` < 13) & (graf_pop$`F(%)` >= graf_pop$`A(%)`)
three_pop <- (graf_pop$`A(%)` < 95) & (graf_pop$`E(%)` < 87) & (graf_pop$`F(%)` < 13) & (graf_pop$`F(%)` < graf_pop$`A(%)`)
pac_idx <- three_pop & (graf_pop$GD1 > (30 * graf_pop$GD4^2 + 1.73))
sas_idx <- three_pop & (graf_pop$GD4 > (5 * (graf_pop$GD1 - 1.69)^2 + 0.042))
lat_am_2_idx <- three_pop & (graf_pop$GD4 < 0) & !pac_idx
other_idx <- (graf_pop$`A(%)` >= 13) & (graf_pop$`F(%)` >= 13)
ancestry[afr_idx] <- "AFR"
ancestry[eur_idx] <- "EUR"
ancestry[eas_idx] <- "EAS"
ancestry[afr_am_idx] <- "AFR_AM"
ancestry[lat_am_1_idx] <- "LAT_AM_1"
ancestry[pac_idx] <- "PAC"
ancestry[sas_idx] <- "SAS"
ancestry[lat_am_2_idx] <- "LAT_AM_2"
ancestry[other_idx] <- "OTHER"
return(ancestry)
}
initial_clst <- fread("/scratch/st-dennisjk-1/wcasazza/delahaye_QC/placenta_regulatory_landscape/RootStudyConsentSet_phs001717.PlacentalRegulation.v1.p1.c1.HMB-IRB-PUB-COL-MDS/genotype_qc/raw_data.clst")
graf_pop_dt <- fread("/scratch/st-dennisjk-1/wcasazza/delahaye_QC/graf_pop_results.txt")
initial_clst %>% mutate(graf_pop = assign_ancestry(graf_pop_dt)) %>% left_join(assigned_ancestry,by=c("V1","V2")) %>% filter(graf_pop != "") %>% dplyr::select(V1,V2,graf_pop)%>% write_delim(paste0(BASE_DIR,"raw_data_assigned_ancestry.clst"), quote=F, col_names = F)
initial_clst %>% mutate(graf_pop = assign_ancestry(graf_pop_dt)) %>% group_by(graf_pop)predicted_ancestry <- fread(paste0(BASE_DIR,"raw_data_assigned_ancestry.clst")) %>% mutate(predicted_ancestry=V3) %>% select(-V3)
pcs_predicted_ancestry <- merge(pcs,predicted_ancestry,by=c("V1","V2"))
plot_pcs(pcs_predicted_ancestry,"V3","V4",1,"","predicted_ancestry")
plot_pcs(pcs_predicted_ancestry,"V4","V5",2,"","predicted_ancestry")
plot_pcs(pcs_predicted_ancestry,"V5","V6",3,"","predicted_ancestry")
plot_pcs(pcs_predicted_ancestry,"V6","V7",4,"","predicted_ancestry")
plot_pcs(pcs_predicted_ancestry,"V7","V8",5,"","predicted_ancestry")
predicted_ancestry_meta <- merge(metadata%>% rename(maternal_ethnicity=Self.declared.race),predicted_ancestry,by.x="SUBJECT_ID",by.y="V2")
table(predicted_ancestry_meta[,c("predicted_ancestry","maternal_ethnicity")]) %>% knitr::kable()| Asian & Pacific Islander | Hispanic | Non-Hispanic Black | Non-Hispanic White | |
|---|---|---|---|---|
| AFR | 0 | 0 | 7 | 0 |
| AFR_AM | 0 | 14 | 64 | 4 |
| EAS | 27 | 0 | 0 | 0 |
| EUR | 0 | 5 | 0 | 67 |
| LAT_AM_1 | 0 | 28 | 1 | 1 |
| LAT_AM_2 | 0 | 43 | 0 | 3 |
| OTHER | 1 | 11 | 1 | 0 |
| PAC | 11 | 1 | 0 | 2 |
| SAS | 11 | 0 | 0 | 0 |
source /home/wcasazza/miniconda3/bin/activate
conda activate snakemake
cd /scratch/st-dennisjk-1/wcasazza/delahaye_QC/placenta_regulatory_landscape/RootStudyConsentSet_phs001717.PlacentalRegulation.v1.p1.c1.HMB-IRB-PUB-COL-MDS/
/arc/project/st-dennisjk-1/software/plink1.9/plink --bfile genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter --keep genotype_qc/raw_data_assigned_ancestry.clst --make-bed --out genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.ancestryPLINK v1.90b6.18 64-bit (16 Jun 2020) www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang GNU General Public License v3
Logging to genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.ancestry.log.
Options in effect:
--bfile genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter
--keep genotype_qc/raw_data_assigned_ancestry.clst
--make-bed
--out genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.ancestry
192030 MB RAM detected; reserving 96015 MB for main workspace.
1826278 variants loaded from .bim file.
277 people (150 males, 127 females) loaded from .fam.
--keep: 277 people remaining.
Using 1 thread (no multithreaded calculations invoked).
Before main variant filters, 277 founders and 0 nonfounders present.
Calculating allele frequencies... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99% done.
Total genotyping rate is 0.997271.
1826278 variants and 277 people pass filters and QC.
Note: No phenotypes present.
--make-bed to
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.ancestry.bed
+
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.ancestry.bim
+
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.ancestry.fam
... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99%done.Batch association
get covariates to associate:
read.delim(paste0(BASE_DIR,"raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.ancestry.fam"),sep="",header=F) %>%
left_join(metadata,by = c("V2"="SUBJECT_ID"))write("NA", paste0(BASE_DIR,"raw_data_sample_batches.txt"))
[Mon Apr 11 16:33:17 2022]
Finished job 0.
1 of 1 steps (100%) done
Complete log: /scratch/st-dennisjk-1/wcasazza/delahaye_QC/placenta_regulatory_landscape/RootStudyConsentSet_phs001717.PlacentalRegulation.v1.p1.c1.HMB-IRB-PUB-COL-MDS/.snakemake/log/2022-04-11T162919.438551.snakemake.loglibrary(qqman)
fnames <- dir(BASE_DIR, pattern = "raw_data.*.assoc.logistic", full.names = TRUE)
reg <- lapply(fnames, function(x)fread(x)[TEST == "ADD"])
batch <- gsub(".*raw_data_(.*).assoc.logistic","\\1",fnames)
names(reg) <-batch
lapply(batch, function(x){
qq(reg[[x]]$P,main=x)
manhattan(na.omit(reg[[x]]),main=x)
})list()Hardy weinberg
PLINK v1.90b6.18 64-bit (16 Jun 2020) www.cog-genomics.org/plink/1.9/
(C) 2005-2020 Shaun Purcell, Christopher Chang GNU General Public License v3
Logging to genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.ancestry.batch.hwe.log.
Options in effect:
--bfile genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.ancestry.batch
--exclude genotype_qc/raw_data_rm_hwe.txt
--make-bed
--out genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.ancestry.batch.hwe
192030 MB RAM detected; reserving 96015 MB for main workspace.
1826278 variants loaded from .bim file.
277 people (150 males, 127 females) loaded from .fam.
--exclude: 1825782 variants remaining.Warning: At least 3 duplicate IDs in --exclude file.Using 1 thread (no multithreaded calculations invoked).
Before main variant filters, 277 founders and 0 nonfounders present.
Calculating allele frequencies... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99% done.
Total genotyping rate is 0.997273.
1825782 variants and 277 people pass filters and QC.
Note: No phenotypes present.
--make-bed to
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.ancestry.batch.hwe.bed
+
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.ancestry.batch.hwe.bim
+
genotype_qc/raw_data.geno.maf.mind.sex_check.het_filter.ibd_filter.ancestry.batch.hwe.fam
... 0%1%2%3%4%5%6%7%8%9%10%11%12%13%14%15%16%17%18%19%20%21%22%23%24%25%26%27%28%29%30%31%32%33%34%35%36%37%38%39%40%41%42%43%44%45%46%47%48%49%50%51%52%53%54%55%56%57%58%59%60%61%62%63%64%65%66%67%68%69%70%71%72%73%74%75%76%77%78%79%80%81%82%83%84%85%86%87%88%89%90%91%92%93%94%95%96%97%98%99%done.
<bound method NDFrame.head of CHR SNP TEST A1 ... GENO O(HET) E(HET) P
0 0 200610-377 ALL(NP) A ... 0/36/46 0.4390 0.3427 0.009085
1 0 kgp22785392 ALL(NP) G ... 15/46/21 0.5610 0.4973 0.373100
2 0 kgp22785396 ALL(NP) G ... 3/26/53 0.3171 0.3141 1.000000
3 0 kgp22785397 ALL(NP) G ... 10/37/35 0.4512 0.4535 1.000000
4 0 kgp22785398 ALL(NP) G ... 0/10/71 0.1235 0.1158 1.000000
... ... ... ... .. ... ... ... ... ...
18262775 26 2010-08-MT-483 ALL(NP) G ... 0/0/11 0.0000 0.0000 1.000000
18262776 26 MitoC16329T ALL(NP) A ... 0/0/11 0.0000 0.0000 1.000000
18262777 26 2010-08-MT-502 ALL(NP) G ... 1/0/10 0.0000 0.0000 1.000000
18262778 26 2010-08-MT-504 ALL(NP) G ... 1/0/10 0.0000 0.0000 1.000000
18262779 26 2010-08-MT-511 ALL(NP) G ... 1/0/10 0.0000 0.0000 1.000000
[18262780 rows x 9 columns]>[Mon Apr 11 16:36:47 2022]
Finished job 0.
1 of 1 steps (100%) done
Complete log: /scratch/st-dennisjk-1/wcasazza/delahaye_QC/placenta_regulatory_landscape/RootStudyConsentSet_phs001717.PlacentalRegulation.v1.p1.c1.HMB-IRB-PUB-COL-MDS/.snakemake/log/2022-04-11T163507.820208.snakemake.logpre-imputation prep
export BCFTOOLS_PLUGINS=/arc/project/st-dennisjk-1/software/bcftools-1.10.2/plugins/
source /home/wcasazza/miniconda3/bin/activate
conda activate snakemake
cd /scratch/st-dennisjk-1/wcasazza/delahaye_QC/placenta_regulatory_landscape/RootStudyConsentSet_phs001717.PlacentalRegulation.v1.p1.c1.HMB-IRB-PUB-COL-MDS/
rm genotype_qc/raw_data*.vcf.gz
snakemake --configfile config.yaml -j20 genotype_qc/raw_data.pre_imputation.vcf.gzPost imputation QC
Concatenate MIS output, check cumulative frequency plot of R2 scores, and filter out variants with an R2 < 0.3:
source /home/wcasazza/miniconda3/bin/activate
conda activate snakemake
cd /scratch/st-dennisjk-1/wcasazza/delahaye_QC/placenta_regulatory_landscape/RootStudyConsentSet_phs001717.PlacentalRegulation.v1.p1.c1.HMB-IRB-PUB-COL-MDS/
snakemake --configfile config.yaml -j16 genotype_qc/all_imputed_r2_30_rsid.vcf.gzMAF matching
source /home/wcasazza/miniconda3/bin/activate
conda activate snakemake
cd /scratch/st-dennisjk-1/wcasazza/delahaye_QC/placenta_regulatory_landscape/RootStudyConsentSet_phs001717.PlacentalRegulation.v1.p1.c1.HMB-IRB-PUB-COL-MDS/
snakemake --configfile config.yaml -j16 genotype_qc/raw_data.imputed.r2_30.maf_mismatch.trawPlotting this matched data out:
kg_freq <- fread(paste0(BASE_DIR,"imputed_freq_dosages_1kg_EUR.afreq"))
my_freq <- fread(paste0(BASE_DIR,"imputed_freq_dosages_EUR.afreq"))
merged_freq <- merge(my_freq,kg_freq,by=c("#CHROM","ID"))
ggplot(merged_freq, aes(ALT_FREQS.x,ALT_FREQS.y)) + geom_bin2d()Batch association
check batch association
read.delim(paste0(BASE_DIR,"all_imputed_r2_30_rsid.sample"),sep =" ") %>%left_join(metadata,by = c("ID_1"="SUBJECT_ID")) %>% filter(ID_1 != 0) %>% write_delim(paste0(BASE_DIR,"imputed_sample_batches.txt"))source /home/wcasazza/miniconda3/bin/activate
conda activate snakemake
cd /scratch/st-dennisjk-1/wcasazza/delahaye_QC/placenta_regulatory_landscape/RootStudyConsentSet_phs001717.PlacentalRegulation.v1.p1.c1.HMB-IRB-PUB-COL-MDS/
snakemake --configfile config.yaml -j20 genotype_qc/raw_data.imputed.r2_30.maf_mismatch.batch.genreg_results <- fread(paste0(BASE_DIR,"imputed_dosage_batch_1.PD1.glm.logistic.hybrid"))
Error in fread(paste0(BASE_DIR, "imputed_dosage_batch_1.PD1.glm.logistic.hybrid")) :
File '/scratch/st-dennisjk-1/wcasazza/delahaye_QC/placenta_regulatory_landscape/RootStudyConsentSet_phs001717.PlacentalRegulation.v1.p1.c1.HMB-IRB-PUB-COL-MDS/genotype_qc/imputed_dosage_batch_1.PD1.glm.logistic.hybrid' does not exist or is non-readable. getwd()=='/scratch/st-dennisjk-1/wcasazza/sex_specific_mQTL/delahaye_all_QC'hard calling
source /home/wcasazza/miniconda3/bin/activate
conda activate snakemake
snakemake --configfile config.yaml -j20 TERRE_QC/raw_data.imputed.r2_30.hard_call.maf_mismatch.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